[精品文档]大麦黄矮病毒合肥分离物的鉴定及CP基因进化分析.pdf

, Journal ofAnhuiAgr.i Sci. 2010, 38( 23): 12520 12522 郑丹丹 况玲玲 CP * 王伟, 刘 艳 , 王锡锋, 吴蓓蕾, 郑传临 (, , 100193) [目的]为了明确合肥地区大麦黄矮病毒株系种 及分子变异情况[方法]利用生物学手段和RTPCR方法鉴定了 2009年采自 合肥地区的 19 个分离物, 并测定了分离物的外壳蛋白基因(CP 基因)的序列[结果]合肥地区的 12 个分离物被鉴定为 BYDVPAV, CP 基因序列比对发现核苷酸一致性为 95. 8% ~ 99. 7%系统进化树分析得知, 12 个合肥分离物序列聚 到一个分支上, 它们与中国分离 物PAVCN(AY 855920)的CP 基因亲缘关系最近[结论]掌握合肥地区大麦黄矮病毒的株系分布及分子变异情况, 对指导该地区小麦 黄矮病的抗性育种和防治工作有着非常重要的意义 大麦黄矮病毒; 外壳蛋白基因; 系统进化树 + S435. 121. 4 9 A 0517 6611(2010) 23 12520 03 Identification of the Barely Yellow DwarfV irus Strain Isolated in Hefei and Analysis of the Evolution of Coat Protein Gene WANGW ei et al ( StateKey Laboratory for B iology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing 100193) A stract [ Objective] The species and molecularvariability of the strain of barely yellow dwarf virus( BYDV) isolated inH efeiwas clarified. [Method] 19 isolates collected in 2009 inH efeiw ere identifiedw ith them ethod of biology and RTPCR, and the sequence of the coat protein gene of the isolateswas tested. [ Results] 12 isolatesw ere identified as BYDVPAV and the unanmi ity of the nucleotidewas 95. 8% 99. 7% comparedw ith theCP gene sequence. Itwas revealed that 12 isolates from H efeiw as clustered into a branch through the analysis of the hylo genetic tree, wh ich was closed to thePAVCN gene(AF216863) of the isolate of Ch ina in genetic relationship. [ Conclusion] The distribution of BYDV strain in H efei and itsm olecular variability were mastered, wh ich was significant in the gu idance to the resistant breed ing and the control of the disease. Key words BYDV; Coat protein gene; Phylogenetic tree ( Barely Y ellow DwarfV iruses, BYDV s) 1. 2 1951 1. 2. 1 (Sh izap his gram inum, Sg) [ 1] , , (S itobion avenae, S