长链非编码RNA CCAT1通过调控线粒体凋亡通路影响结肠癌细胞增殖的机制研究.docVIP

精品论文 参考文献 长链非编码RNA CCAT1通过调控线粒体凋亡通路影响结肠癌细胞增殖的机制研究 （复旦大学生命科学学院 200030） 【摘要】目的：本文旨在明确Lnc RNA CCAT1是否通过调控线粒体凋亡通路影响结肠癌细胞增殖。方法：首先通过RT-PCR测定结肠癌癌组织与癌旁组织及多种结肠癌细胞系中CCAT1的表达量，然后设计合成si-Scramble、si-CATT1分别感染结肠癌细胞SW480，测定感染及对照组的细胞增殖能力、凋亡情况及细胞周期，并应用蛋白印迹法分析与线粒体凋亡通路相关的基因Caspase-3、Caspase-9、Bax和Bcl-2的蛋白表达。结果：CCAT1在结肠癌癌组织及结肠癌细胞中的表达显著高于癌旁组织及正常细胞，敲除CATT1后的结肠癌细胞活力下降，凋亡数增加、细胞周期阻滞在G2/M期。敲除CCAT1的癌细胞中Caspase-3、Caspase-9、Bax的表达显著高于对照组，而Bcl-2明显低于对照组。结论：长链非编码RNA CCAT1能通过调控线粒体凋亡通路影响结肠癌细胞增殖。 【关键词】 结肠癌；Lnc RNA CCAT1；线粒体；凋亡；增殖 【中图分类号】R73 【文献标识码】A 【文章编号】2095-1752（2015）35-0371-04 The molecular study of Lnc RNA CCAT1 influences colon cancer cell proliferation by regulating mitochondrial apoptotic pathway 【Abstract】Objective: To confirm whether Lnc RNA CCAT1 influence colon cancer cell proliferation by regulating mitochondrial apoptotic pathway. Methods: First, mRNA levels of Lnc RNA CCAT1 in cancer tissues，paratumor tissues and many colon cancer cell lines were determined. Then, si-Scramble and si-CATT1 were designed synthetized and transfected on colon cancer cell SW480. Cell proliferation, apoptosis and cell cycle of SW480 after transfection and in the control were determined. Western blot assay was used to determine protein expression of mitochondrial apoptotic pathway related gene Caspase-3、Caspase-9、Bax and Bcl-2. Results: mRNA level of Lnc RNA CCAT1 in cancer tissue and colon cancer cell lines were significantly higher than paratumor tissues and the normal cells. For SW480 with knockdown of CATT1, the cell viability decreased and apoptosis rate increased markedly, cell cycle was resisted at G2/M. Protein expression of Caspase-3、Caspase-9 and Bax in cell SW480 with knockdown of CATT1 were much higher than the control and si-Scramble group, which the expression of Bcl-2 was much lower. Conclusion: Lnc RNA CCAT1 can influence colon cancer cell proliferation by regulating mitochondrial apoptotic pathway. 【Key words】Colon cancer; Lnc RNA CCAT1；Mitochondria