高效液相色谱法测定人血浆中奋乃静的浓度.docVIP

精品论文 参考文献 高效液相色谱法测定人血浆中奋乃静的浓度 陈清霞 王丽霞 刘伟忠 （中山大学附属第二医院药学部 广东广州 510120） 【摘要】目的 建立测定人血浆中奋乃静浓度的反相高效液相色谱法。方法　以钻石C18反相柱(150mmtimes;4.6mm, 5micro;m)为色谱柱，流动相为0.03molbull;L-1醋酸铵-甲醇（23:77）；流速：1.0 mlbull;min-1；柱温：40deg;C; 检测波长: 254nm。以乙酸乙酯与二氯甲烷(80:20)为提取剂。结果　奋乃静的高、中、低（1000.0, 500.0，10.0micro;g.L-1）3种浓度平均回收率分别为104.28%, 97.60%，98.40%，日内、日间差RSD均低于7%（n=5）; 分析方法的检测限为5.0micro;gbull;L-1；线性范围为10.0-1000.0micro;gbull;L-1。回归方程为:C=39.42F+2.56，r=0.9995（n=10）。结论　该方法灵敏、准确、简单、快速，可用于临床血药浓度监测和药动学研究。 【关键词】奋乃静 血药浓度 反相高效液相色谱法 【中图分类号】R927.2 【文献标识码】A 【文章编号】2095-1752（2013）25-0038-02 Determination of the concentration of perphenazine in plasma by RP-HPLC with UV detection CHEN Qing-xia Wang li-xia LIU Wei-zhong (Department of pharmic ,The Second Affiliated Hospital, Sun Yat-sen University, Guangzhou510120,China.) 【ABSTRACT】 Objective To establish a method for determining the plasma concentration of perphenazine by RP-HPLC Methods Blood plasma samples containing the drug were extracted with hexane for the removal of lipids and other interfering substances and thereafter extracted with ethyl acetate. The extract was then analyzed in a reverse phase HPLC system ( C18 column, 150mmtimes;4.6mm, 5micro;m); mobile phase consisted of 0.03molbull;L-1NH4AC-methanol (23:77); The detection wavelength was at 254nm. Results The average recoveries of perphenazine in high, mediun and low concentrations(1000.0, 500.0, 10micro;gbull;L-1) were 104.28%, 97.60% and 98.40%, respectively. The within-day and between-day precision of variation was less than 6%. The calibration curves of perphenazine had good linearity, r=0.9995（n=10）, over the range of 10.0-1000.0micro;gbull;L-1 .The regression equation was C=39.42F+2.56, r=0.9996(n=9). The minumum detectable concentration of perphenazine was 5.0micro;gbull;L-1. Conclusion The method provides a sensitive, accurate precise and reliable analytical procedure for clinical monitoring of perphenazine in plasma and it