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蓝莓根癌病发生调查及病原鉴定 Investigation and Identification of Blueberry Crown Gall
吉林农业大学学报2011,33(3):283—286,292 http://xuebao.Ⅳ口u.edu.cn
Joumul
ofJilin
AgriculturalUniversity
蓝莓根癌病发生调查及病原鉴定
傅俊范1,彭 超1,严雪瑞1,周如军1,代汉萍2
1.沈阳农业大学植物保护学院,沈阳110866;2.沈lZa农业大学园艺学院,沈阳110866
摘 要:对辽宁、吉林、山东及黑龙江省的部分蓝莓苗圃及生产园中蓝莓根癌病发生情况、症状特点及危害程
度进行了调查,结果表明蓝莓根癌病多见于苗圃及酸度不适的生产园中。在上述地区采集到57份蓝莓冠瘿
瘤和lO份土壤样品,经过菌株分离、回接寄主后获得19株致病菌。通过菌落形态、菌体特征、生理生化及16S
rDNA序列比对,确定蓝莓根癌瘸是由根癌土壤杆菌(Agrobacteriumtumefacienj)侵染所致。
关键词:蓝莓;根癌病;病原菌鉴定;16SrDNA序列分析;根癌土壤杆菌
中图分类号:S663.9;s436.639文献标识码:A 文章编号:1000.5684(20II)03.0283.04
DOl:CNⅪ:22一1100/S0835.005
网络出版地址:http://www.cnki.net/kems/detaii/22.1100.S0835.005.html
引文格式:傅俊范,彭超,严雪瑞,等.蓝莓根癌病发生调查及病原鉴定[J].吉林农业大学学报,2011。33(3):
283.286,292.
and
Identificationof CrownGall
Investigation Blueberry
FU
dun—fanl,PENGCha01,YANXue—ruil,ZHOURu—junl,DAIHan.pin92
Plant 110866,China;2.Col—
of
1.College Protection,ShenyangAgriculturalUniversity,Shenyang
of 110866,China
legeHorticulture,ShenyangAgriculturalUniversity,Shenyang
Abstract:Thecrowndiseaseoccurrencewas indifferent nurseriesandfields
gall investigated blueberry
in
located and seven andtensoil
Liaoning,Jilin,ShandongHeilongjiangprovinces.Fiftyplantsamples
were attheaboveareas.Nineteenof of
collected themshowed
samples pathogenicitythroughinoculating
was featuresofthe
healthyblueberry.Thepathogenidentified髂Agrobacteriumtumefaciensbyphenotypic
cellsand characteristicsand of16SrDNA.
colonies,biochemical sequenceanalysis
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