Chromatin immunoprecipitation ChIP of plant transcription factors followed by sequencing ChIP SEQ or hybridization to whole genome arrays ChIP CHIP英文文献.pdfVIP

protocol Chromatin immunoprecipitation (ChIP) of plant transcription factors followed by sequencing (ChIP-SEQ) or hybridization to whole genome arrays (ChIP-CHIP) 1 2 3 3 4 5, 6 Kerstin Kaufmann , Jose M Muiño , Magne Østerås , Laurent Farinelli , Pawel Krajewski Gerco C Angenent 1 2 Laboratory of Molecular Biology, Wageningen University, Wageningen, The Netherlands. Applied Bioinformatics, Plant Research International, Wageningen University s l 3 4 o Research Centre, Wageningen, The Netherlands. Fasteris SA, Plan-les-Ouates, Geneva, Switzerland. Institute of Plant Genetics, Polish Academy of Sciences, c 5 6 o Poznań, Poland. Bioscience, Plant Research International, Wageningen, The Netherlands. Centre for BioSystems Genomics (CBSG), Wageningen, The Netherlands. t Correspondence should be addressed to K.K. (kerstin.kaufmann@wur.nl). o r p e r Published online 18 February 2010; doi:10.1038/nprot.2009.244 u t a n / chromatin immunoprecipitation (chIp) is a powerful technique to study interactions between transcription factors (tFs) and Dna in vivo. m o For genome-wide de novo discovery of tF-binding sites, the Dna that is obtained in chIp experiments needs to be processed for sequence c . e identification. the sequences can be identified by direct sequencing (chIp-seQ) or hybridization to microarrays