A fluorosensor for two-dimensional measurements of extracellular enzyme activity推荐.pdfVIP

Marine Chemistry 123 (2011) 23–31 Contents lists available at ScienceDirect Marine Chemistry journal homepage: www. elsevi /locate/marchem A fluorosensor for two-dimensional measurements of extracellular enzyme activity in marine sediments Zhenrui Cao, Qingzhi Zhu, Robert C. Aller ⁎, Josephine Y. Aller School of Marine and Atmospheric Sciences, Stony Brook University, Stony Brook, NY 11794-5000, United States a r t i c l e i n f o a b s t r a c t Article history: A novel planar fluorosensor has been developed for measuring two-dimensional activity distributions of the Received 22 May 2010 extracellular enzyme, leucine-aminopetidase (Leu-AP), in marine sediments. The sensor is a foil consisting of Received in revised form 24 August 2010 an enzyme substrate L-leucine 7-amido-4-methylcoumarin hydrochloride (Leu-MCA) and a Hydromed D4 Accepted 8 September 2010 membrane, backed by a polyester sheet. In its present application, the sensor foil is introduced into surficial Available online 18 September 2010 deposits to a depth of ~10–15 cm, and time dependent fluorescence production occurring in successive digital images is used to resolve enzyme activity patterns. Corrections for microenvironmental variability (e.g., Keywords: Enzyme planar fluorosensor diffusion and adsorption properties) and background reflectance were made. The planar fluorosensor Extracel