加速衰老小鼠脑组织中的衰老相关基因的表达.PDFVIP

50(4) : 600- 607, 2004 A ct a Zoolog ica S in ica * 1, 2 1 3 1** 张 冲 程锦雁 王锦刚 陈清轩 1. , 100080 2. , 100039 3. , 100086 , DDRTPCR SAM ( Se esce ceaccelerated mouse) SAMR1TASAMP8/ TaSAMP10/ Ta , SAMP10/ T a (241218 ) , 4 12 ; SAMP8/ Ta (2411 ) , 2 11 16 , SAMP10/ Ta ( 3 ) SAMP8/ T a ( 6 ) SAMR1TA ( 7 ) , : 70AT P RNA Dleu mRNAX RP23334C4 DNA Qc 72 kD 60 S L21FIS9 1/ PER 1 CGNAP 2 DNA prkdc [ 50 ( 4) : 600- 607, 2004] * Expressions of agingassociated gene in cerebral tissue of senescenceaccelerated mouse 1, 2 1 3 1** ZHANG Cho g , CHENG Ji Ya , WANG Ji Ga g , CHEN Qi gXua 1. I stitute of Ge etics a d Developme tal Biology, The Chi ese Academy of Scie ces, Beiji g 100080, Chi a 2. Graduate School of Chi ese Academy of Scie ces, Beiji g 100039, Chi a 3. Li gtai Biche Medical T ech ology Co. Ltd. Beiji g 100086, Chi a Abstract A better u dersta di g of the molecular effects of agi g i t he brai may help to reveal importa t aspects of or ga ismal agi g, as well as processes that lead to agi grelated brai dysfu ctio . I this study, the agei gspecific expres sio ge es of the muri e cerebrum were i vestigated by employi g differe tialdisplay reverse tra script io polymerase chai reactio ( DDRTPCR) i three se esce ceaccelerated mouse ( SAM ) strai s: SAMP8/ Ta, SAMP10/ T a a d SAMR1TA. Through compared ge e expressio profile amo g the age 18, 12, 4, a d 2 mo th of the SAMP10/ Ta strai , two differe tial fragme ts have bee fou d, which belo g to 4 a d 12 mo t h i dividually; a d i age 11, 4 a d 2 mo th of the SAM P8/ Ta strai , two fragme ts have bee fou d, w hich