新血府逐瘀汤对动脉粥样硬化大鼠主动脉斑块形成及th1 2fth2失衡的影响.docxVIP

提要目的观察新血府逐瘀汤对动脉粥样硬化（Atherosclerosis ,AS）模型大鼠胸主动脉 AS 斑块形成及血清中辅助性 T 细胞 1（Th1）/辅助性 T 细胞 2（Th2）细胞因子失衡的影响，探讨其防治 AS 的机制。方法选用 SPF 级雄性 Wister 大鼠 65 只随机分为 5 组，分别为空白组、模型组、中药低剂量组、中药高剂量组、西药组。除空白组外，其它各组均采用高脂饲料喂养联合维生素 D3 腹腔注射的方法复制 AS 模型。实验开始时即给予中药组中药灌胃，给予西药组立普妥加蒸馏水稀释后灌胃。药物干预组每周称重 1 次，根据体重调整用药量。整个实验周期为 8 周。8 周后腹主动脉取血测量大鼠血清中细胞因子 IFN-r 及 IL-4的含量，计算 IFN-r/IL-4 的比值。取胸主动脉 3-5cm,经固定、染色后，镜下观察胸主动脉病理学改变，并测量其内膜及中膜的厚度。结果 与模型组相比，药物组主动脉内膜及中膜厚度下降，主动脉病理改善，差异有统计学意义（P0.05），大鼠血清中 IFN-r 含量降低、IL-4 含量升高，IFN-r/IL-4比值降低，Th1/Th2 细胞功能失衡向 Th2 偏移，差异有统计学意义（P0.05）。结论新血府逐瘀汤可通过调节细胞中 T 细胞亚群 Th1/Th2 细胞的分化失衡，抑制 AS 过程中的免疫炎性反应，预防 AS 的形成。关键词: 新血府逐瘀汤；动脉粥样硬化；Th1 细胞；Th2 细胞Effect of Renewed Xuefuzhuyu Decoction on the formation of Aorticplaque and the imbalance of Th1/Th2 Cytokine in AtherosclerosisRatsSpeciality:Traditional chinese medicineAuthor: Wang YongTutor: Ji ZhongqiangAbstractObjective：To observe the effect of Renewed Xuefuzhuyu Decoction on theformation of Aortic plaque and the imbalance of Th1/Th2 Cytokine in AS rats ,andresearch the mechanism of Renewed Xuefuzhuyu Decoction preventing AS.Methods： 65 SPF Wister male rats were randomly divided into five groups: Theblank group, model group, Chinese medicine in low dose group and high dose group,western medicine group. Except the blank group, other groups were made into AS modelby feeding high-fat diet and injecting vitamin D3 into abdominal cavity. The drug wasgiven through irrigation stomach in each group. After 8 weeks ,IFN-r、IL-4 in serum wasmeasured by using ELISA, then the ratio of IFN-r / IL-4 will be calculated. The intima andmedia thickness of Thoracic aortic was measured through Microscope, and then observethe change of Thoracic aortic pathology through microscope.Result：Xuefuzhuyu Decoction can decrease the thickness of the intima and mediaof Thoracic aortic ,can improve the Aortic pathology. Campared with the model group,thecontent of IFN-r in serum was reduced, and IFN-r in serum was increased. the function ofTh1 / Th2 cell offset to the Th2 .Conclus