补中益气丸补气疗效的谱-效关系研究δ-中国药房.pdfVIP

·中药研究· 补中益气丸补气疗效的谱-效关系研究Δ ＊ # 胡 芳 ，杨英来，刘小花，朱瑞娟，李 灿，封士兰 （兰州大学药学院，兰州 730000） 中图分类号 R285；R927 文献标志码 A 文章编号 1001-0408（2014）03-0195-04 DOI 10.6039/j.issn. 1001-0408.2014.03.02 摘 要 目的：探讨补中益气丸高效液相色谱（HPLC ）指纹图谱与其补气作用的关联性，确立其补气药效成分。方法：建立补中益 气丸的HPLC-二极管阵列检测器（DAD ）和HPLC-蒸发光散射检测器（ELSD ）指纹图谱；用控食法和疲劳法联合复制小鼠气虚模 型，测定小鼠体质量、脏器指数、廓清指数和矫正廓清指数；用灰关联度分析方法研究其谱-效关系。结果：HPLC-DAD 指纹图谱中 各峰显示橙皮苷（峰号11）、芒柄花素（峰号19）对补气作用有较大贡献。HPLC-ELSD 指纹图谱中各峰显示橙皮苷（峰号4）对补气 作用有较大贡献。结论：补中益气丸对模型小鼠气虚症状有明显改善作用；HPLC-DAD 和HPLC-ELSD 指纹图谱均与补中益气丸 的补气作用有一定对应关系；补中益气丸中对补气作用贡献较大的成分是橙皮苷、芒柄花素。 关键词 补中益气丸；指纹图谱；补气疗效；灰关联度；谱-效关系 Spectrum-effect Relationship of Tonifying Qi Efficacy of Buzhong Yiqi Pills HU Fang ，YANG Ying-lai ，LIU Xiao-hua ，ZHU Rui-juan ，LI Can ，FENG Shi-lan （School of Pharmacy ，Lanzhou University ，Lanzhou 730000，China ） ABSTRACT OBJECTIVE ：To analyze the correlation between HPLC fingerprints of Buzhong yiqi pills and tonifying qi efficacy ， and to establish its medicinal components group of tonifying qi efficacy. METHODS ：HPLC-DAD and HPLC-ELSD fingerprint of Buzhong yiqi pills were established ；qi deficiency mice model was induced by controlling diet and fatigue test ；body weight ，vis- cera index ，clearance index and correct clearance index were determined. The spectrum-effect relationship was studied using gray re- lational grade. RESULTS ：The tonifying qi efficacy of each peak in HPLC-DAD fingerprint were hesperidin （peak 11）and for- mononetin （peak 19）. The tonifying qi efficacy of each peak in HPLC-ELSD fingerprint was hesperidin（peak 4）. CONCLUSIONS ： Buzhong yiqi pills have obvious improvement for qi deficiency symptoms of mice. There is certain corresponding relation between HPLC-DAD ，HPLC-ELSD fingerprint and tonifying qi efficacy of Buzhong yiqi pills. Hesperidin ，formononetin in Buzhong Yiqi pills is big role in buqi effection. K