Detectors for High Performance Liquid Chromatography高性能液相色谱检测器.pptVIP

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Detectors for High Performance Liquid Chromatography高性能液相色谱检测器.ppt

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Detectors for High Performance Liquid Chromatography高性能液相色谱检测器.ppt

* * * * * * * Dunnivant * * * * * * * * * * * * * * * * * * * * * * * * * * * /chromedia?waxtrapp=dwobkOsHqnOxmOlIEcCbCoFhE * * Carbonyl, C=O * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * Chromatographic Methods…Common Detectors for Chromatography Detectors for High Performance Liquid Chromatography Limited compared to GC detectors: UV detectors Fluorescence detectors Refractive index detectors Chromatographic Methods…Common Detectors for Chromatography Detectors for High Performance Liquid Chromatography UV Detectors Relies on strong absorbance of analyte in UV region Same UV detector as used in UV-VIS spectrometers Most powerful UV detectors are photodiode array (PDA) detectors Simultaneous detection of spectral data in a range of wavelengths Chromatographic Methods…Common Detectors for Chromatography Detectors for High Performance Liquid Chromatography Fluorescence Detectors Design similar to UV-VIS units Requires two wavelengths of emitted radiation UV lamp emits a range of wavelengths Filters or monochromator acquires the needed exciting beam λexcitation Emitted light (fluorescence) is measured at a 90o angle to the exciting light beam at a wavelength λemission Chromatographic Methods…Common Detectors for Chromatography Detectors for High Performance Liquid Chromatography Fluorescence Detectors Not universal, limited to certain chemicals Typically 1 order of magnitude more sensitive than UV Most intense fluorescence is found in aromatic compounds e.g. PAHs Chromatographic Methods…Common Detectors for Chromatography Detectors for High Performance Liquid Chromatography Refractive Index Detectors Refractive index of solutions is changed by the presence of solutes RI detector responds to all solutes (universal) Chromatographic Methods…Common Detectors for Chromatography Detectors for High Performance Liquid Chromatograph