ace2ang17mas轴调控的胰岛内皮细胞保护胰岛β细胞的效应及机制分析 word格式.docx

本研究由国家自然科学基金“糖尿病前期胰岛内皮细胞功能的调控机制”（No资助目录英文缩略词表1前言4第一部分：ACE2 基因敲除加重高脂诱导的糖尿病前期小鼠胰岛Β细胞损害中文摘要10ABSTRACT12材料与方法14结果23讨论30结论34参考文献35第二部分：ANG(1-7)对生理及高脂培养条件下胰岛内皮细胞功能的影响及机制研究中文摘要39ABSTRACT40材料与方法42结果51讨论61结论66参考文献67第三部分：ANG(1-7)介导的胰岛内皮细胞功能对B细胞的调节效应及机制研究. 中文摘要72ABSTRACT73材料与方法75结果79讨论86结论89参考文献90综述94参考文献99附录：攻读博士学位期间发表的文章104致谢105英文缩略词表RASrenin-angiotensinsystem肾素血管紧张素系统ACE2angiotensin-converting enzyme 2血管紧张素转换酶2Ang(1-7)angiotensin(1-7)血管紧张素(1-7)ACEangiotensin-converting enzyme血管紧张素转换酶AngIIangiotensinII血管紧张素IIAT1Rtype 1 angiotensin II receptor血管紧张素II 受体1ACEIangiotensin-converting enzyme inhibitor血管紧张素转换酶抑制剂ARBtype 1 angiotensin II receptor blocker血管紧张素II受体1阻断剂DMDiabetes Mellitus糖尿病WTwild type野生型KOknockout基因敲除IEQisletsequivalent quantity胰岛当量IRCinsulin relative concentration细胞内胰岛素相对浓度ICDinsulin-positive cell density胰岛素阳性细胞密度VEGFvessel endotheliumgrowth factor血管内皮生长因子iNOSinduced nitic oxide synthase诱导型一氧化氮合酶IL-1βinterleukin-1β白细胞介素1βTNF-αtumor necrosis factor –α肿瘤坏死因子GSISglucose stimulatedinsulin secretion葡萄糖刺激的胰岛素分泌mRNAmessenger RNA信使RNAMCP-1monocyte chemotactic protein-1细胞趋化蛋白-1siRNASmall interfering RNA小分子干扰RNAFFAfree fattyacid游离脂肪酸PA eNOS NOpalmitateendothelial nitric oxide synthase nitric oxide棕榈酸内皮型一氧化氮合酶一氧化氮PI3KAkt/PKB MAPK JNK HGFTSP-1 CAMs PGI ET ICAMVCAMNF-KBphosphatidylinositol 3-kinase proteinkinase Bmitogen-activated protein kinases c-Jun N-terminal kinase hepatocyte growth factor thrombospondin 1cellularadhesion molecules prostaglandinendothelinintercellular adhesionmolecule vascular cell adhesionprotein1 nuclear factor kappa B磷脂酰肌醇-3-激酶蛋白激酶B 促分裂素原活化蛋白激酶c-Jun氨基末端激酶肝细胞生长因子凝血酶敏感素-1 血浆细胞黏附分子前列腺素内皮素细胞间粘附分子血管细胞粘附分子核因子κBROSreactive oxygen species活性氧族cDNADMEMcomplementary DNADulbeccomodified Eagle medium互补DNADulbecco改良Eagle培养基PBS DMSO DEPC DPI NADPHPVDF TBS BSA ECL PI DAPIphosphate buffered saline dimathyl sulfoxidediethyl pyrocarbonate diphenyleneiodoniumchloride reduced formofnicotinamide adenine dinucleotide phosphate polyvinylidenefluorideTris-HClbuffer sali