9.4t离体磁共振频谱对间充质干细胞生物标识及成脂分化的分析 word格式.docxVIP

intracellular metabolites, such as choline compounds, creatine, glutamate and acetate all decreased, with the increased level of methionine, succinate and fatty acids after the MSCs differentiation 2 weeks. Intracellular choline, acetate, glutamate and creatine reduced from 6.3 ± 0.68, 0.97 ± 0.23, 0.3± 0.05 and 0.1 ± 0.02 mM to 1.1 ± 0.06 (p0.01), 0.45 ± 0.1 (p0.01),0.16 ± 0.08 mM (p0.05) and no-detected, respectively. Inversely, the methionine, succinate and fatty acids (1.28 ppm) increased from 0.03 ± 0.01, 0.11 ± 0.02, (1.18±0.07)n mM (here, n is the number of CH2) to 0.12 ± 0.05 (p0.01) and 0.15±0.05 (p0.05) and (1.18±0.07)n mM (p0.01). After 14 daysdifferentiation, the cells shape transformed into ovoid/round morphology and many lipid droplets distributed in the cytoplasm appeared jacinth with the red-O staining. In addition, the mRNA expression of adipocyte-specific genes, such as peroxisome proliferator-activated receptor (PPAR-r), acyl-CoA synthetase (ACS), and the enzyme lipoprotein lipase (LPL) in the MSCs were increased obviously.Conclusion: The biomarker features of MSCs were obtained by using high resolution MR spectroscopy. The 1.28ppm biomarker considered as unique for NPCs was also observed in the MSCs spectra, but it is not specific biomarker for the stem cells. MR spectroscopy is capable of monitoring the adipogenic differentiation of the MSCs, according to the metabolites change.Keywords: MR spectroscopy, mesenchymal stem cells, adipogenic differentiation, biomarker主要缩略词中英文对照英文缩写MSCs英文全名mesenchymal stem cells中文译名间充质干细胞MRSmagnetic resonance spectroscopy磁共振频谱MRImagnetic resonance imaging磁共振成像NPCsneural progenitor cells神经干祖细胞DMEMDulbecco’s modified Eagle’s mediumDMEM 培养基FBSfetal bovine serum胎牛血清PBSphosphate buffered saline磷酸盐缓冲液PCAperchloric acid高氯酸PCRploymerase chain reaction聚合酶链式反应Laclactate乳酸Aceacetate醋酸Glugluatmate谷氨酸Crcreatine肌酸Chocholine胆碱Pcphosphocholine磷酸胆碱Gpcglyocerphosphocholine甘油磷酸胆碱Myomyo-Inosito肌醇Tautaurine牛磺酸Methmethionine