9顺式视黄酸抑制wntβcatenin信号通路调节胆管癌qbc939细胞的迁移和侵袭的研究 word格式.docxVIP

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9顺式视黄酸抑制wntβcatenin信号通路调节胆管癌qbc939细胞的迁移和侵袭的研究 word格式.docx

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9顺式视黄酸抑制wntβcatenin信号通路调节胆管癌qbc939细胞的迁移和侵袭的研究 word格式

目录目录 ······································································································ I 中文摘要······························································································· II 英文摘要······························································································ III 第一章前言 1第二章实验材料·····················································································6 细胞株································································································6 主要仪器及设备 ···················································································6 主要试剂·····························································································7 主要溶液的配制 ················································································· 10质粒与菌种 ······················································································· 11第三章实验方法··················································································· 12感受态细胞的制备与转化 ···································································· 12细胞培养··························································································· 12质粒的提取 ······················································································· 12质粒转染··························································································· 13Western Blot 检测基因蛋白水平表达15细胞总蛋白质的提取··········································································· 15SDS电泳15Western Blot 分析16RNA 提取16RNA 反转录17荧光定量 PCR 实验（Real -Time PCR 实验）18引物设计··························································································· 19 细胞划痕实验（Wounded hell) ······························································ 19 细胞侵袭实验(transwell) ······································································ 20 细胞毒性实验（MTT 法） ··································································· 20 统计学方法 ············································································