gmcsf凝胶对深ⅱ度烧伤创面溶痂的影响及其机理分析 word格式.docxVIP

EffectsofRecombinantHumanGranulocyte-Macrophage Colony-Stimulating Factor on Debridement of Deep Partial-Thickness BurnGraduate:LIU Ji-songSupervisor:FANG YongBackground and ObjectiveEschar adheres to the deep partial-thickness wound causes a negative effect during burn wound healing. Due to the persistence of necrotic tissue (eschar) the local inflammation and infection become extensive and profound, which may leads to a delayed healing. Therefore, early removal of eschar by using tangential excision or Chinese medicine has shown a great therapeutic benefit in the deep partial-thickness burn.GM-CSF is synthesized and secreted by a variety of inflammatory cells (neutrophils and macrophages) and repair cells during wound healing. In turn, GM-CSF chemoattracts inflammatory cells into the wound site and induces productions of matrix metalloproteinases (MMPs). Previous studies showed that proteolytic enzymes and MMPs play a major role in debridement of eschar of deep partial thickness burn. Thus, we hypothesize that GM-CSF could deride the eschar of deep partial-thickness burn so that could speed up the process of wound healing.The propose of this study is to determine the effect of rhGM- CSF on debridement of eschar in deep partial thickness scalding wound and the mechanism for debridement of rhGM- CSF using animal experiments and clinical observations.Methods：1．Animal experiments：A total of 70 SD rats were scalded on the back using the method of thermal-water burns at 75°C for 8s. A deep partial-thickness burn with diameter of 3cm per rat was created in all the rats. Scalded rats wererandomly divided into two groups（n=35）, namely, control group(C) andexperiment group(E). Control group(C) was treated with gel matrix ( without rhGM-CSF), experiment group(E) was treated with rhGM-CSFgel（100μg/10g）. The wounds were treated with rhGM-CSF or gel matrixup to 21 day. Observations of the wounds were made by photograph at various time-points. In addition, the removal ti