鳖甲煎丸对多种肝损伤实验模型中mda的影响及机制探讨的实验研究-方剂学专业论文 word格式.docxVIP

河南中医学院硕士学位论文鳖甲煎丸对多种肝损伤实验模型中MDA的影响及机制探讨的实验 研究姓名：任周新申请学位级别：硕士专业：方剂学指导教师：谢世要[目的]:探讨鳖甲煎丸对肝内过氧化脂质及其清除系统的影响。[方法 J :采 用 CC14腹腔注射的方法复制小鼠肝损伤模型，同时给予药物，比较各组肝组织匀 浆中 MDA 的差异:分离药物血清，制作 CC14 损伤小鼠的肝组织匀浆，二者共培 养，观察药物血清对肝组织匀浆中 MDA 的影响:分离、纯化大鼠肝细胞，制成 培养液，用 CCJ4 复制肝细胞损伤的体外模型，同时给予药物血清，观察药物血清 对培养液中 MDA 、SOD、GSH、ALT 的影响。实验中，设有 0.5、10.、16.、2.S6g/kg 体重 4个药物剂量组，以了解剂量的影响。[结果]:CC14 腹腔注射复制肝损伤的 同时，给予 2.56gl陆的鳖甲煎丸能显著抑制肝组织匀浆中 MDA 的升高 CPO.Ol )0 1.6g/kg 的药物血清与 CC14 小鼠的肝组织匀浆共培养，与生理盐水血清组比较， MDA 含量显著降低 (PO.Ol )。鳖甲煎丸含药血清对CCl4 攻击的肝细胞的影响与 剂量有关，在 1.0 1.6g/kg 范围内，能够显著降低 ALT 、MDA 值 CPO.Ol )0增 加 GSH 水平，但是在 2.56g1kg 剂量下， GSH 水平及 SOD 活力显著下降，而 ALT、 MDA 显著升高( PO.05-0.01)0[结论]:在一定的剂量范围内，鳖甲煎丸对 CCl4 致肝细胞损伤有保护作用，这种作用与提高肝细胞的自由基的清除能力有关。但当剂量过高时，却对肝细胞有损伤作用。提示，在应用时，应注意对剂量的把握。 关键词z鳖甲煎丸; CC14 ;肝损伤; MDA; SOD; GSH; ALTExperinlentalStudy on Effect and Its Mechanismin MDA of Some Liver Lnjury Models with Bie Ji勾ian PillsAbstract[Objective]: To study effects of BieJiaJian Pi11. on antioxidation in hepatic tissue and cells. [Methods]: CC14 oily solution was injected into abdomen cavity of mice for duplicated hepatic injury anímal model ，some groups were given different dose ofBieJiaJianpillandMDAlevelsinthehepatictissueofdifferentgroupswere compared each other. Mice were given Biejiajian pills事 and their serum were taken and liver tissue was taken fromCC14 modelmice.The serum was addedinto the tissues andcultureforsometime.Theeffectofserumincludingthepillon m lonyldialdehyde(MDA) was detected.Hepatic cells were takenandpurifiedformatra and were made ?nto culture medium ，then were injured by CC14 s01uton to makethe model of hepatic cel1ular injury. At tbe same time，serum with or without drug was.given into the cu1ture medium ，after some time，SOD(superoxide dismutase) ，ALT activityand MDA ，GSH (glutathione) concentration in the medium were detected. In the experiment ，O.久1.0，2.56g/kg BJJ pill doses were made. IResults]: The MDA level in hepatic tissue of 2.56g/kg BJJ pill dose group was dramtically lower t