分析限制性内切酶的识别和切割序列.pptVIP

One common use in the dairy industry is as a marker of pasteurisation in cows milk. This molecule is denatured by elevated temperatures found during pasteurisation, and can be tested for via colour change of a para-nitro-phenol phosphate substrate in a buffered solution. Raw milk would typically produce a yellow colouration within a couple of minutes, whereas properly pasteurised milk should show no change. 用途： （1）使缺失5`-P末端的DNA发生磷酸化作用。 （2）标记DNA的5`-末端； 用途： (1) 主要用于给外源DNA片段和及载体分子加上互补同聚物尾巴； (2) 标记DNA片段的3`末端。 1953年，Arber研究噬菌体与细菌（A、B）的关系。 发现100-200个子代噬菌体中，只有1个可感染B，即其他的噬菌体在B中受到限制，唯有这一个受到修饰（甲基