关于肉羊qtl最新研究进展.pptVIP

Confirmed linkage for QTLs affecting muscling in Texel sheep on chromosomes 2 and 18 G.A. Walling, P.M. Visscher, G. Simm S.C. Bishop Roslin Institute (Edinburgh) University of Edinburgh Scottish Agricultural College * Project Aims Started July 1999 Detect and verify QTL for growth and carcass traits Investigate genotyping strategies http://www.roslin.ac.uk/sheepmap * Aims Use commercial UK Texel sheep Use candidate regions to Determine evidence for major genes: Chromosome 2 Chromosome 18 * Scientific Background Studies in Belgium, Australia and New Zealand suggest a major gene for muscling and fatness on chromosome 2 Putative Callipyge and Carwell loci both increase muscling and are located on chromosome 18 * Animals Five large half-sib families 1999: 348 progeny 2000: 275 progeny Blood collected from all rams and progeny DNA extracted from blood * Phenotypic Traits 8 Week Weight (8WW) Weight at ultrasonic scanning (ScanWT) Ultrasonic Muscle Depth (Mus) Ultrasonic Fat Depth (Fat) Muscle Depth adjusted for weight (MusWT) Fat Depth adjusted for weight (FatWT) * Genotyping All 5 sires screened for: 28 markers on chromosome 2 14 markers on chromosome 18 Progeny genotyped for markers heterozygous in their sire * Linkage Maps Produced using Cri-Map Final maps in good agreement with previous studies * Statistical Analysis Traits corrected for fixed effects and covariates: estimated using ASREML Inheritance probabilities calculated at 1cM intervals Regress adjusted phenotypes on inheritance probabilities at each location for each family * Thresholds and Further Analyses Nominal P-value of 0.01: Lander Kruglyak (1995) Significant results: Compare with a 2 QTL model using grid search method Confidence intervals using bootstrapping * Results: Chromosome 2 Significant effects clustered around two regions: Fat traits around 170cM Muscle traits around 65cM Best model for weight traits was a QTL in each of these two regions: P(2QTL vs. 1QTL) 0.05 * Ch