脉冲射频能量prfe加快糖尿病小鼠伤口愈合的研究-血管外科专业论文.docx

独创性声明本人郑重声明，本学位论文是本人在导师指导下进行的研究工作及取得的研究成 果的总结。尽我所知，除文中已经标明引用的内容外，本论文不包含任何其他个人或 集体已经发表或撰写过的研究成果。对本文的研究做出贡献的个人和集体，均已在文 中以明确方式标明。本人完全意识到本人将承担本声明引起的一切法律后果。学位论文作者签名：日期：年月日学位论文版权使用授权书本学位论文作者完全了解学校有关保留、使用学位论文的规定，即：学校有权保 留并向国家有关部门或机构送交论文的复印件和电子版，允许论文被查阅和借阅。本 人授权华中科技大学可以将本学位论文的全部或部分内容编入有关数据库进行检索，可以采用影印、缩印或扫描等复制手段保存和汇编本学位论文。本论文属于保密□，在年解密后适用本授权书。不保密□。（请在以上方框内打“√” ）学位论文作者签名：指导教师签名：日期：年月日日期：年月日目录摘要················································································································ 1ABSTRACT31 绪言··········································································································52 材料和方法······························································································ 52.1 动物和伤口模型················································································52.2 脉冲射频能量治疗············································································ 62.3 图像采集与计算················································································72.4 组织学································································································72.5 免疫组织化学····················································································82.6 细胞增殖的定量················································································82.7 血管增殖的定量················································································82.8RNA提取与cDNA生成92.9 胶原纤维排列··················································································103.0 统计分析·························································································· 103 结果········································································································103.1 伤口愈合速度··················································································103.2肉芽组织··························································································113.3 伤口胶原沉积和分布···························································