dha减轻脂多糖介导的新生大鼠脑白质损伤分析word格式论文.docxVIP

open-field activityor or suckling. The mean birth weight of the LPS-treated group neonatal rats was significant lower than the DHA-treated group (p0.05). Placental hematoxylin-eosin staining showed obvious inflammatory response in the LPS-treated group. Inflammatory infiltration of the placental in the DHA-treated group was decreased. MBP staining was clearly observed in hippocampus of P7 neonatal brain, LPS-treated groupwas much weaker than in the control group（1.73±0.35* vs. 2.15±0.53，p0.05）；MBPstaining was continue expressed in hippocampus of P14 neonatal brain, LPS-treated group was much weaker than in the control group（2.18±0.56* vs. 2.52±0.36，p0.05）；but GFAP staining in the LPS-treated group was more than in the control group in hippocampus and subcortex white matter of P7 neonatal brain (hippocampus 1.96±0.54* vs. 1.23±0.35，p0.05；subcortex white matter 2.17±0.24* vs 1.53±0.11 p0.05）. GFAP-positive atrocities were observed in cortex and subcortex white matter of P14 neonatal rat brain, the number of GFAP-positive atrocities were more increasing in the LPS-treated group than in the controlgroup(cortex 1.89±0.31* vs. 0.83±0.25，p0.05；subcortex white matter 2.32±0.39* vs.±0.24，p0.05). MBP staining of the DHA-treated group was much more than of the LPS-treated group in the same age ( p0.05). GFAP staining of the DHA-treated group was much weaker than of the LPS-treated group in the same age(p0.05). Real-time PCRshowed general average of IL-1? ?mRNA ?and TNF-αmRNA in LPS-treated group weresignificantly increasing than in NS-treated group (p0.05); DHA treated group were significantly decreasing than in LPS-treated group (p0.05); DHA treated group were significantly increasing than in control group (p0.05). But there was distinction at different time point.Conclusions Intrauterine LPS infection could induce an inflammatory response of placenta and fetus, and resulted in neonatal low birth weight and WMD, which characterized by astrogliosis and hypomyelination. DHA wa