dickkopf1蛋白与经典wnt信号通路失活在帕金森病模型中的作用分析word格式论文.docx

2 方法 ·········································································· 473 结果 ·········································································· 494 讨论 ·········································································· 55参考文献 ······································································ 58全文总结 ········································································· 60 综 述 帕金森病发病机制的研究进展 ····································· 62 附 录 ·············································································· 96 致 谢 ·············································································· 97缩写词表英文缩写英文全称中文名称MPP+1-methyl-4-phenylpyridinium1-甲基-4-苯基吡啶离子 PDParkinson’s Disease帕金森病MTT3-(4,5-dimethylthiazol-2-yl)-2,5-diphen yltetrazolium bromide四甲基偶氮唑蓝DMSOdimethyl sulfoxide二甲基亚砜GSK-3βglycogen synthase kinase-3β糖原合成酶激酶-3βECLenhanced chemiluminescence增强化学发光THtyrosine hydroxylase酪氨酸羟化酶ANOVAanalysis of variance方差分析MPTP1-methyl-4-phenyl-1,2,3,6-tetrahydropy ridine1-甲基-4-苯基-1,2,3,6-四氢吡啶6-OHDA6-hydroxydopamine6-羟多巴胺FBSfetal bovine serum胎牛血清PMSFphenymethylsulfonyl fluoride苯甲基磺酰氟 GAPDHglyceraldehyde 3-phosphate3-磷酸甘油醛脱氢酶 siRNAsmall interfering RNA小干扰 RNABSAalbumin from bovine serum牛血清白蛋白SDSsodium dodecyl sulfate十二烷基硫酸钠PBSphosphate buffer saline磷酸盐缓冲液APammonium persulfate过硫酸胺Tris2-amino-2-(hydroxymethyl)-1,3-propan ediol三羟甲基氨基甲烷Acracrylamide丙烯酰胺Bicbis-acrylamide甲叉双丙烯酰胺TBStris buffer salineTris 缓冲盐溶液Glyglycine甘氨酸ODoptical density吸光度PSphosphatidylserine磷脂酰丝氨酸PIpropidine iodide碘化丙啶FITCfluorescein isothiocyanate异硫氰酸荧光素TUNELterminaldeoxynucleotidyltransferase mediated nick end labeling脱氧核糖核苷酸末端转移酶 介导的缺口末端标记法TdTterminal deoxynucleotidyl transferase脱氧核糖核苷酸末端转移酶HRPperxidase(horseradish)辣根过氧化酶DABdiaminobenzidine二氨基联苯胺TEMEDN,N,N,N-tetramethylethylenediamineN,N,N,N-四甲基乙二胺 ADAlzheimers disease阿尔茨海默病rhDkk1recombinant Human Dkk1重组人 Dkk1DAPI4,6-diamidino-2-phenylindole4,6-二脒基-2-苯基吲哚 RNAiRNA interferenceRNA 干扰MFBmedial forebrain bundle内侧前脑束MAO-Bmonoamine oxidas