dna电化学生物传感器的构建及评价双酚a 丙烯酰胺对dna损伤的分析word格式论文.docxVIP

DNA电化学生物传感器的构建及评价双酚A、丙烯酰胺对DNA损伤的研究liquid-Nafion modified pyrolyticgraphite electrode(PGE) wasprepared, and thenhorseradishperoxidase(HRP)andnaturaldouble-strandedDNAwerealternatelyassembledonthemodifiedelectrodebythelayer-by-layermethod.ThePGE/graphene-ionicliquid-Nafionandtheconstructionofthe(HRP/DNA)nfilmwerecharacterizedby electrochemicalimpedancespectroscopy.WiththeguaninesignalinDNAasanindicator,thedamageofDNAwasdetectedbydifferentialpulsevoltammetryafterPGE/graphene-ionicliquid-Nafion/(HRP/DNA)nwasincubatedinAAsolutionorAA+H2O2solutionat37oC.ThismethodprovidesanewmodeltomimicanddirectlydetectDNAdamageinducedby chemicalpollutantsandtheirmetabolitesinvitro.Theresultsindicatedthat,inthepresenceofH2O2,HRPwasactivatedandcatalyzedthetransformationofAAtoglycidamide,whichcouldformDNAadductsandinducemoreseriousdamageofDNAthanAA.Inordertofurtherverifytheseresults,UV-visspectrophotometrywasalsousedtoinvestigateDNA damageinducedbyAAanditsmetabolitesinsolutionandthesimilarresultswereobtained.Keywords:Electrochemicaldetection;DNAdamage;Acrylamide;BisphenolA; Horseradish peroxidase; Layer-by-layer; MWNT-Chit nanocomposite4符号说明CV:cyclicvoltammetry,循环伏安法Chit:chitosan,壳聚糖DNA:deoxyribonucleicacid,脱氧核糖核酸dsDNA:doublestrandedDNA,双链DNA ssDNA:singlestrandedDNA,单链DNADPV:differentialpulsevoltammetry,示差脉冲伏安法EDC:1-[3-(dimethylamino)propyl]-3-ethylcarbodiimidehydrochloride,1-乙基-3-(3-二甲基氨丙基)碳二亚胺盐酸盐EIS:electrochemicalimpedancespectroscopy,电化学阻抗法ks:chargetransferconstant,电荷转移常数MWNT:multi-walledcarbonnanotube,多壁碳纳米管NHS:N-hydroxysulfosuccinimide,N-羟基琥珀酰亚胺PAMAM:polyamidoamine,聚酰胺-胺PBS:phosphatebuffersolution,磷酸盐缓冲液R:linearcorrelation,线性相关系数SCE:saturatedcalomelelectrode,饱和甘汞电极GCE:glassycarbonelectrode,玻碳电极PGE:pyrolyticgraphiteelectrode,热解石墨电极BPA:bisphenolA,双酚A AA:acrylamide,丙烯酰胺GA:glycidamide,环氧丙烯酰胺HRP:horseradishperoxidase,辣根过氧化物酶IL:ionicliquid,离子液体关于学位论文原创性和使用授权的声明本人所呈交的学位论文，是在导师指导下，独立进行科学研究所取得的成果。对在论文研究期间给予指导、帮助和做出重要贡献的个人或集体，均在文中明确说明。本声明的法律责任由本人承担。本人完全了解山东农业大学有关保留和使用学位论文的规定，同意学校保留和按要求向国家有关部门