肝复康对ccl 4所致的肝纤维化大鼠肝组织tgfβ 1和collagen ⅰ的影响word格式论文.docxVIP

Experimental Study of Effect on Hepatic Tissue TGFβ1 and collagen -Ⅰof Hepatic Fibrosis in Rats by GFKAbstractObjective: To observe the effect on hepatic tissue transforming growth factorβ1 (TGFβ1) and type Ⅰcollagen (collagen-Ⅰ) of hepatic fibrosis in rats by GFK, and further discuss the mechanism of anti-action and the appropriate dosage.Methods: The models of hepatic fibrosis in rats were established by hypodermic injected with tetrachloride carbon and drinking the alcohol to rats. Each rat in treatment, which wasgiven respectively to GFK of 6.25g、12.5g、25g/per kilogram weight at the same time.After 8 weeks of treatment, all the rats were executed and their livers were obtained for the content of hepatic tissue TGFβ1 and collagen-Ⅰ were detected by immunohistochemical technique.Results:TGFβ1 and collagen-Ⅰ of the model group increased significant, which comparedwith the normal group(p﹤0.05). The low GFK group has no obvious difference, which compared with the model group(p﹥0.05). The content of hepatic tissue TGFβ1 depressedsignificant in the medium GFK group and the high GFK group and the FFBJRGP group, which compared with the model group(p ﹤ 0.05);and the content of hepatic tissue collagen-Ⅰ depressed obviously (p﹤0.05).Conclusions:1. The GFK can reduce hepatic tissue TGFβ1 of hepatic fibrosis in rats and reduce deposition of hepatic tissue collagen-Ⅰ,and depress extracellular matrix(ECM)ofhepatic fibrosis tissues. 2. In the medium GFK group and the high GFK group and the FFBJRGP group, which anti-hepatic fibrosis function has no obvious difference.3. The GFK has the function to treatment hepatic fibrosis, and with the dosage scope.Key wor ds： GFK； Hepat i c Fi br osi s； TGFβ1； col l agen- Ⅰ； I HAut hor ： Tut or ：陕西中医学院学位论文独创性声明及使用授权声明学位论文独创性声明本人郑重声明：所呈交的学位论文，是个人在导师的指导下，独立进行研究工作 所取得的成果，无抄袭及编造行为。除文中已经特别加以注明引用的内容外，本论文 不含任何其他个人或集体已经发表或撰写过的作品成果。对本文的研究做出重要贡献 的个人和集体，均已在文中以明确方式标明并致谢。本人完全意识到本声明的法律结 果并由本人所承担的法律责任。学位论文作者签名＿＿＿＿＿ 日期：年月日关于学位论文使用授权的声明陕西中医学院有权保留使用本人学位论文，同意