卵巢恶性上皮性肿瘤中HLA.docVIP

卵巢恶性上皮性肿瘤中HLA 【关键词】 卵巢癌 摘 要 目的：探讨卵巢癌中HLADR抗原表达和树突细胞浸润的情况及其意义。方法：用免疫组化SP法对卵巢恶性上皮性肿瘤(48例)、良性上皮性肿瘤(30例)及正常卵巢(30例)的HLADR抗原表达及树突细胞浸润情况进行对照研究。结果：正常卵巢组织中HLADR阴性,卵巢恶性上皮性肿瘤中HLADR表达阳性率为52.08％(P＜0.01)；HLADR表达率与肿瘤分化程度相关(P＜0.01)，且与预后相关(P＜0.05)；卵巢恶性上皮性肿瘤中树突细胞(S100+DC)浸润显著增加(P＜0.001)；S100+DC浸润与肿瘤间质淋巴细胞浸润相关(P＜0.01)；S100+DC浸润与肿瘤患者预后相关(P＜0.05)。结论：卵巢恶性上皮性肿瘤中HLADR抗原表达增强，树突细胞浸润增加，二者可能是局部抗肿瘤免疫的重要环节；HLADR和树突细胞浸润数量可作为提示卵巢癌患者预后的指标。 关键词 卵巢癌;预后;HLA抗原/免疫学;S100蛋白;树突细胞;免疫组织化学 XIAO Guohong, CHEN Huizhen,ZHANG Guiqing,ZHENG Xiuling Second Municipal Peoples Hospital,GuangZhou，Guangdong 510150 Abstract Objective:To investigate the clinical and immunological significance of HLADR antigen expression and dendritic cell infiltration in epithelial ovarian cancer. Method: Samples of 108 cases including normal ovarian tissue (n=30),benign (n=30) and malignant epithelial ovarian tumor (n=48) were used in a comparative study. The SP immunohistochemical stain method was used.Result:HLADR antigen positive rate was significantly higher in the malignant group (52.08%) than that in benign group (10%) and in normal group(0%) (P＜0.01). There was a correlation between the HLADR expression rate and the tumuor differentiation and between HLADR expression and the prognosis. The number of infiltrating dendritic cells (S100+DC) was much higher in malignant(538.17±255.01) than that in benign group(345.96±226.81) and in normal group (335.15±160.32) (P＜0.01). There was a correlation between the dendritic cell (S100+ DC) infiltration and the intertumour lymphocyte infiltration (P＜0.01) and the prognosis (P＜0.05).Conclusion:It was shown that HLADR antigen expression and dendritic cells infiltration in epitherial ovarian cancer increased, which could play a important role in antitumour immunity. The expression of HLADR antigen and the grade of dendritic cells infiltration in epitherial ovarian cancer is the indicator for the prognosis. Key words ovarian cancer;HLADR antigen/immundogy;S100 protein;dendritic cell;prognosis;immu