胎盘提取液影响脂蛋白-胆固醇代谢的实验研究.docVIP

胎盘提取液影响脂蛋白-胆固醇代谢的实验研究 中国临床药理学与治疗学 2000年第2期第5卷 论著 作者：崔文姬　杨景文　吕忠智 单位：崔文姬（天津市第一中心医院,天津 300192）；杨景文（白求恩医科大学第一临床学院心内科）；吕忠智（白求恩医科大学基础药理研究室） 关键词：人胎盘提取液；脂蛋白-胆固醇代谢；过氧化脂质；超氧化物歧化酶；前列环素；血小板聚集率 　　目的　探讨人胎盘提取液对高脂血症大鼠的脂蛋白-胆固醇代谢、过氧化作用及血小板聚集功能的影响及其作用机制。方法　建立Wistar大鼠高血脂模型,用胎盘提取液0.4 ml·(100 g·d)-1连续灌胃12 d,测定其血清TG 、TC、LDL-C 、HDL-C及其亚组分HDL 2-C的含量；测定全血及肝组织LPO、SOD的活性,并通过脂肪染色观察提取液对肝脏脂肪沉积的影响；通过放免法测定血浆中6-酮-PGF1α、TXB 2含量及血小板最大聚集率。结果　胎盘提取液使高脂鼠HDL-C、HDL2-C含量明显升高(P＜0.01);TG、TC、LDL-C含量明显降低(P＜0.01)；明显降低高脂鼠血清及肝组织LPO含量(P＜0.01),提高SOD活性(P＜0.01)；抑制肝脏脂肪沉积,使脂肪空泡明显减少；使血浆中PGI2含量明显升高(P＜0.01),TXB2含量无明显变化,并明显降低血小板聚集率(P＜0.01)。结论　胎盘提取液明显改善高脂大鼠脂蛋白-胆固醇代谢,增强机体抗氧化能力,抑制血小板聚集,并抑制脂肪肝形成,有利于拮抗动脉粥样硬化的发生和发展。 　　中图分类号　R965 Effect of human placental extract 　　on the lipoprotein-cholesterol metabolism CUI Wen-Ji 　　(Tianjin First Central Hospital, Tianjin 300192) 　　YANG Jing-Wen 　　(Tianjin First Central Hospital, Tianjin 300192) 　　LV Zhong-Zhi 　　(Tianjin First Central Hospital, Tianjin 300192) 　　Aim　The effect and mechanism of human placental extract(HPE) on the lipoprotein-cholesterol metabolism, peroxidation and the function of platelet aggregation in hyperlipaemia rats were abserved.Methods　Wistar rat with hyperlipaemia models were given each HPE 0.4 ml (100 g)-1.d-1 through lavage for 12 days.The serum levels of TG,TC,LDL-C,HDL-C and HDL2-C in its subgroup were measured.The activies of LPO and SOD in both blood and liver tissue were determined .The effect of HPE on lipidosis of liver were abserved by fat dyeing.The levels of 6-keto-PGF1α,TXB2 in plasma and maximum platelet aggregation rate were measured by ELASA. Result　The levels of HDL-C and HDL2-C were increased (P＜0.01) while the levels of TG, TC and LDL-C were decreased (P＜0.01).The levels of LPO in serum and liver tissue decreased obviously and SOD activity increased (P＜0.01).The lipidosis in liver was inhibited and adipose hollow space reduced obviously.The levels of PGI2 in plasma increased (P＜0.01),the level of TXB2 was not changed and platelet aggregation rate wa