结肠炎癌变过程中muc1粘蛋白变化及苹果多糖对muc1影响-changes of mucin in mu c1 during colitis carcinogenesis and effects of apple polysaccharide on mu c1.docxVIP

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结肠炎癌变过程中muc1粘蛋白变化及苹果多糖对muc1影响-changes of mucin in mu c1 during colitis carcinogenesis and effects of apple polysaccharide on mu c1.docx

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结肠炎癌变过程中muc1粘蛋白变化及苹果多糖对muc1影响-changes of mucin in mu c1 during colitis carcinogenesis and effects of apple polysaccharide on mu c1

Variation of MUC1 in the process of colitis associatedcolon carcinogenesis and the effect of apple polysaccharides on MUC1 expressionAbstractObjective: MUC1, a high molecular weight and high glycosylated transmembrane protein, highly expressed in tumor tissue, and is closely related to the occurrence, development and metastasis of tumor. MUC1 as an important marker of tumor biology has drawn more and more attention. In this study, apple polysaccharides were extractedfrom apple pomace, and colon cancer cell lines SW-1116, HT-29, and colitis-associated colon cancer mice model were used to observe the effect of apple polysaccharide on MUC1 and its preventive effect on colitis carcinogenesis; study the changes of MUC1 in colitis carcinogenesis process, and provide a theoretical basis for MUC1 as a potential therapeutic target for colitis associated colorectal cancer prevention.Methods: The methods of water extraction, ethanol precipitation, enzyme degradation, and dialysis were used to extract polysaccharides from pomace; Colon cancer cell lines SW- 1116 and HT-29, were treated with 0.5 mg / mL, 1mg/mL apple polysaccharide (AP) for 24 and48 h. Proteins were then extracted, and Western-blot was used to detect the MUC1 expression. Carcinogenic agent azoxymethane (AOM) and proinflammatory agent dextran sodium sulfate (DSS) were applied orderly to copy colitis associated colon cancer mouse model. One hundred and fifty five-week-old male ICR mice were randomly divided into five groups, namely, control group, model group, 3 dose of AP group (n = 30). At the beginning of the experiment, the control group mice were given saline only, and the rest mice were injected intraperitoneally with a single dose of 10mg/kg AOM. One week later, the mice were given 2.5% (w/v) DSS in their drinking water for 1 week. This was followed by no further treatment for 1 week. After another week of 2.5% DSS treatment, normal water was given for an additional 16 week. On week 7, the mice in AP-treate