解淀粉芽孢杆菌y19微生物菌肥的研制及其生物效益分析-preparation and biological benefit analysis of bacillus amyloliquefaciens y19 microbial fertilizer.docxVIP

萝卜、白菜和油菜。试验结果表明，单一的 Y19 发酵液与基质复配促生长效果最好。5. 将Y19 发酵液（106CFU/mL）与不同含量的氮肥、磷肥或钾肥的基质均 匀混合后，用于漂浮育苗水稻和盆栽玉米的促生试验。结果发现Y19 发酵液与含 有 3~4%的磷肥的基质复配的促生长效果最好。盆栽 30 d后，与对照相比，玉米 鲜重可增加 448.05%-487.60%。这表明Y19 可与含有化学肥料的基质复配且制成 的复合基质能显著地促进玉米幼苗生长发育。6. 玉米根际土壤微生物群落功能样性及土壤微生物量的变化检测结果发现 施入 Y19 微生物菌肥后，玉米根系细菌、放线菌的数量增加，而真菌的数量却 下降，特别是镰刀菌的数量。此外，Y19 微生物菌肥也提高了根际微生物群落的 碳源利用率（AWCD)，主要提高了对碳水化合物类、氨基酸和羧酸类化合物的 利用。这些数据说明 Y19 微生物菌肥可通过改善根际土壤微环境，从而发挥促 生和防病的双重功能。7. Y19 微生物菌肥菌株货架期检测结果发现Y19 在纯基质及含 3%磷肥的基 质中存活率最高。在温度为 28 ℃、湿度为 50%的环境条件下保存 1 年，其活菌 量维持在 107CFU/g以上。关键词：内生菌；促生长防病机能；微生物菌肥；货架期；定殖Development of Microbial Fertilizer and Biological Effect ofBacillus amyloliquefaciens Y19 Candidate: JIANG Xiaoling Directed by Prof. HE YueqiuAbstractCurrently, the increase of crop yields and protection from pests and diseases mainly rely on excessive application of chemical fertilizer and pesticide in agricultural production. However, it also arises a series of problems at the same time, such as environment pollution , disruption of ecological balance and food safety. Besides the reduction of the harmness of diseases and pests, microbial fertilizer has been demonstrated to improve crop production in previous research. Therefore, it is of great significance to carry out scientific research on screening beneficial microorganism and developing them into the microbial fertilizer. The selection of biocontrol strains with growth promotion, microbial ferteilizer development and their biological effects have been conducted in this study, and the main obtained results are as follows:To screen endophytic bacteria with a good application potential, IAA biosynthesis, nitrogen-fixing and phosphate solubilizing activity of maize endophytic bacteria isolated and stored in our lab as well as chlorophyll content in maize leaves after treated with bacteria had been determined. The results showed that Bacillus amyloliquefaciens Y19 strain has a better performance in IAA biosynthesis and nitrogen fixation and phosphate solubilization, and the