辣椒抗疫病相关基因的研究及qtl定位-study on phytophthora blight related genes and qtl mapping in capsicum annuum l..docxVIP

列经BLAST分析，获现弓1物B和弓}物C所扩增的片段大多数与植物的抗 病性状有关，且与番茄、马铃薯、烟草的抗病基因或抗瘸基因同源序列有 较高的一致饿。3．一端用锚定引物oligo(dT)l sA、oligo(dT)l 5C、oligo(dT)l 5G，另一端用根据 已克隆的植物抗病基因的保守区域设计而成的简并引物B，、C，、D，，用 mRNA差异最示技术，分析诱导型辣椒抗疫病相关基因的表达。得到了两 个有豢异表达的片段oligo(dT)l 5CDl和oligo(dT)l 5ADl。经BLAST分析， 在基因库中找到了oligo(dT)l 5cDl片段有较商的同源性的一个番茄的BAC 克隆，该克隆为番茄的一个表达序列标签。4．以我曩融质资源抗瘸晶拳申93．100-17-1．0、感病晶静茄门的杂交厝代建立了 藿内第一个用子分板辣椒拨疫痰相关基因的F2群体，通过遗传分柝和卡 方检验，涯实杂交嚣代豹抗疫瘸性状莠不德会3：l豹分离规咎，裘明 93，100．17．i，0静菰疫瘸性状是壶寡基因或多基因掰控制麴。采建AFLP分 子标诡，耥建了～个含46今标记键煮、ll令连锁群、覆盖长度必1381．5eM、 每个标记闻的平殇距离是30．OcM酌国内第二张辣椒分予连铰鼙谱。弊在 此基础上，褥到多个与辣椒抗疫病稳状稚关静QTL位点，将这些位赢定 位在第1、第2、第5、第7、第8条连锁群上。遂些位赢均能解释表黧交 异率达64％以上。其中最有意义的是位于第5条逐锁群上128～1 32cM阉 的QTL位点，从感受性、诱鲁稳定性这3个方面进行QTL分析均能证实 该位点的存在，区间约为4cM，且能解释表型变异率68％左右。关键词：辣椒痰病；抗病基因；mRNA差异显示；遗传作图；QTL分析llAbstractPhytophthora blight caused by Phytophthora capsici L．is orie of the most economically destructive soil borne diseases of pepper(Capsicum annuum L．) throughout the world and can be a limiting factor for pepper production in tropical areas with hot and humid climates．Symptoms associated with the disease include damping—off,fruit rot，foliar blight，root and crown rot and stem lesion． The pathogen can survive in soil for a long time．The temperature and humidity suitable for the pepper growth is also for the pathogenic fungus．The use of resistant cultivars is the most promising method to control the disease，because the pathogen is easy to counteract the active site of currently used fungicides． However，most scientists believe that there is no pepper variety immune to尸hytophthora capsici．Inheritance of the resistance，a quantitative trait，is very complex and controlled by polygenes．The attainment of pepper cultivars resistant to Phytophthora capsici is difficult because of the polygenic nature of this trait． It is an obstacle to earmark and map the related resistant gene to Phytophthora capsici in pepper for molecular marker·assisted selection．Qie．men，a native susceptible sweet pepper lines，and 93一100-17-1-0，a native resistant pungent