利用uplc-ms研究纳米四氧化三铁颗粒染毒大鼠的毒理学及汽车内饰中的有机磷阻燃剂检测方法-study on toxicology of rats exposed to nano-sized ferroferric oxide particles and detection method of organophosphorus flame retardants in automobile interior by uplc - ms.docxVIP

北京化工大学硕士论文中的八种有机磷阻燃剂的方法。先通过单因素实验考察了对萃取回收率影响较大的几个因素(萃取剂的种类、萃取剂的用量、微波萃取的温度及微波萃取的时间)，并且确定了大致的范围，而后通过BBD设计及响应曲面分析考察了各因素之间的相互作用，并得到了丙酮／乙酸乙酯(3／1，v～)作为最佳的萃取剂。在此基础上也完成对实验方法学的考察，最后成功的将其运用在实际样品的检测中。此外实验中还采取了工业模拟添加的方式进行标准添加样品的制各，使之与实际样品更为贴近。使数据的结果更加可靠。关键词：脂质代谢组学，纳米四氧化三铁，有机磷阻燃剂，液质联用Ⅱ万方数据ABSTRACTLipidomicsAnalysisofSerumfromRatsInfectedbyNanoFerroferricOxideParticlesABSTRACTNanomaterialstechnologyhavetherapiddevelopmentinrecentyears，theirapplicationfieldsandevenextendedtomedical，textile，informationtechnologyandenergy,ere．Nanomaterials，especiallyforitsextraordinarypropertiesgainingincreasingattentionontheapplicationandbasicresearchintheareasofmedicalimaging，diseasediagnosis，drugdeliveryandcancertreatment．Butbeforetheclinicalapplicationofthenanometermaterials，thepossiblebiologicaltoxicityofthetissueshouldhaveasystematizedresearchtoavoidharmfuleffecttohumanbodyhealththanitbringstothebenefitofhumansociety．Thusitisnecessarytohavearesearchofsafetyevaluation，qualitycontrolandtoxicologyforthematerial．Atpresent，thisstudyisfocusedonthedetectionofbiochemicalindexesandhistopathologysection，butthereisnostudyonthetoxicityofendogenoussubstances．Themainachievementsofthispaperareasfollows：1．ThisexperimentresearchobjectisFe304nanoparticleswhicharewidelyusedinbiologyandmedicine．ThemaleWistarratswereHI万方数据北京化工大学硕士论文exposuredbyintraperitonealinjection，theaccumulationofliversurfaceafterexposureofratswithnanoparticleswasfound．Andinaconsiderableperiodoftimeitcannotberuledoutinvitro．Thebiochemicalindexesofserumweredetected，Itwasfoundthat廿lecomemofTGinthemodelgroupwassignificantlyhigherthantllatofcontrolgroup．Thetotalcholesteroldidnotchangesignificantlyintheearlystage，andthenthecomentdecreasedwiththeincreaseofexposuretime．Methanol／chloroformsolventWasusedforthetargetlipidextraction，UPLCWasusedforseparation，QTofwasusedfordetection，theanalysisoflipidWasfinishedin10minutes．AfterthePretreatmentoftherawdata,theanalysisofPCAandPLS—DAwasconducted．AccordingtotheresultsobtainedbyPCAandPLS—DA，theobviousdifferences