藜芦根内放线菌筛选 抑菌活性初探及plantactinospora veratri新种鉴定-preliminary study on screening antibacterial activity of actinomycetes in veratrum and identification of new species of plantactinospora vera tri.docxVIP

CONTENTSCONTENTSAbstract in Chinese一lAbstract in English．．III1 Introduction．11．1 Outline of endophytel1．2 Function ofplant endophyte·ll。2．1 Agricultural function21．2．2 Environment function．．41．2．3 Pharmaceutical function．51．3 Black false hellebore(Vertrum nigrum L．)91．3．1 Pharmacological and toxicological properties of Vertrum nigrum．．9 1．3．2 Diversity of compounds from Veratrum nigrum 101．4 Research purpose and contents．101．4．1 Research purpose and significance．101．4．2 Contents．．1 11．5 Research source．．1 l2 Materials and Methods ．．．．．．．．．．．122．1 Materials．122．1．1 Plant samplesl：12．1．2 Indicator strains ofactivity tests122．2 Reagents and equipments1 32．3 The main mediums and methods ofpreparation142．3．1 Isolation medium．142．：；．2 Purification medium．．142．3．3 Fermentation medium142．3．4 Mycelium medium．142．3．5 E coli culture medium．152．3．6 Medium of activity test．1 52．3．7 Physiological and biochemical assay medium152．3．8 Mediums ofactinomycetes morphology．．15 2．3．9 Main reagents162．4 Isolation and purification of endophytic actinomycetes of Veratrum nigrum．．16 2．4．1 Collection and pretreatment1 62．4．2 Isolation and purification of Veratrum nigrum endophytic actinomycetes172．5 Detection ofthe biological antibacterial activity．172．5．1 Pretreatment ofthe fermentation broth．172．5．2 Detection offermentation antibacterial activity17III万方数据东北农业大学理学硕士学位论文2．5．3 Dectection of actinomycetes colonies activity～1 82．6 Taxonomic identification ofnew species．182．6．1 Charactrization of morphology and culture．1 82．6．2 Detection of physiological and biochemical characteristics一192．6．3 Chemical classification and identification．．20 2．6．4 Identification of the molecular Ievel．．．．．243 Results and Analysis．．．．． 。．。．． 。 。．。．。 。。。．．． 28 3．1 Veratrum nigrum symbiotic actinomycetes isolation．．28：I．2 Determination ofantibacterial activity．．．．29 3．2．1 Detection of indicator bacteria activity．293．2．2 Detection ofanti—fungus activity．293．3 Identification analysis ofNEAU—FHS4．313．3．1 Sequencing and