金属配合物与l-半胱氨酸 dna相互作用的荧光性质分析及应用-fluorescence property analysis and application of interaction between metal complexes and l - cysteine dna.docxVIP

AbstractFor cysteine detection, the mechanism between small molecule fluorescent probe and nucleic acid, the quick method of nucleic acid detection, the papers main work is summarized as follows:In Britton-Robinson buffer solution of pH 7.5, Cu2+ could form the Cu2+-calceincomplex, calcein fluorescence quenched. When Zn2+ was added, the fluorescence intensity increased a little. However, the presence of cysteine, owing to cysteine could reduce Cu2+ to Cu+. Meanwhile, the release of calcein from the complex could combine with Zn2+ and the fluorescence increased. So we established a new fluorescence method of cysteinedetection. Under the optimum conditions, the increase in signal intensity was linear the range from 3.0×10-7 to 1.2×10-5 mol L-1 and the limit of detection was 4.0×10-8 mol L-1. The method was successfully applied to the determination of cysteine in human serum samples and the recovery was satisfactory.the fluorescence and absorption spectra of DNA-Tb3+-PUFX were studied by usingTb3+-PUFX fluorescent probe. The results indicated that the fluorescence intensity of Tb3+-PUFX could be greatly enhanced by DNA, and the fluorescence intensity was in proportion to the concentration of DNA. Accordingly, a new method of DNA detection was established and the fluorescence enhancement mechanism was discussed. Under the optimum conditions, the dynamic ranges of hsDNA and ctDNA were 3.0×10-9—1.0×10-6 g mL-1, 5.0×10-9—8.0×10-7 g mL-1 and the limits of detection were 2.1×10-9 g mL-1, 2.9×10-9 g mL-1, respectively. The method was applied to the DNA determination in synthetic samples and the result was satisfactory.The interaction between Cu2+-PUFX and DNA was investigated, using the Neutral Red dye as a spectral probe by UV-vis absorption and fluorescence spectroscopy. The result indicated that Cu2+-PUFX complex could bind to DNA and the major binding mode was intercalative binding. The binding constants K and number of binding sites n ofCu2+-PUFX complex with DNA and t