金丝桃苷通过bmp和wntβ-catenin信号通路调控成骨细胞mc3t3-e1增殖分化的实验研究-experimental study on hyperoside regulating proliferation and differentiation of osteoblasts mc 3t3 - e1 through bmp and wnt β - catenin signaling pathway.docxVIP

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2018-05-29 发布于上海
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金丝桃苷通过bmp和wntβ-catenin信号通路调控成骨细胞mc3t3-e1增殖分化的实验研究-experimental study on hyperoside regulating proliferation and differentiation of osteoblasts mc 3t3 - e1 through bmp and wnt β - catenin signaling pathway.docx

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金丝桃苷通过bmp和wntβ-catenin信号通路调控成骨细胞mc3t3-e1增殖分化的实验研究-experimental study on hyperoside regulating proliferation and differentiation of osteoblasts mc 3t3 - e1 through bmp and wnt β - catenin signaling pathway

AbstractthathyperosidewillpromotetheexpressionofBMP2proteinandβ-cateninprotein.Especiallyitindicatesthatthehyperosidewiththeconcentrationupto40μmol/Lhasthemostsignificanteffect.Conclusion：HyperosidewillaccelerateosteoblastproliferationanddifferentiationviabothoftheBMPandWnt/β-cateninsignalingpathwaysinMC3T3-E1cells.Keyword：Hyperoside；Osteoblastdifferentiation；BMP；Wnt/β-catenin目录第1章前言····················································································1第2章材料与方法···········································································42.1实验材料···············································································42.1.1主要实验仪器·································································42.1.2实验细胞·······································································52.1.3实验试剂·······································································52.1.4实验药物·······································································62.1.5主要溶液及试剂配制························································62.2实验方法···············································································82.2.1实验器材准备·································································82.2.2实验药物的配置及分组·····················································82.2.3MC3T3-E1细胞培养，传代，冻存，复苏82.2.4MTT法检测细胞增殖率92.2.5p-NPP法检测细胞碱性磷酸酶活性102.2.6茜素红染色法检测矿化结节·············································10RT-PCR检测细胞mRNA的表达11Westernblot检测MC3T3-E1细胞蛋白的表达142.3统计学处理···········································································16第3章结果···················································································183.1MC3T3-E1细胞的形态观察······················································183.2金丝桃苷对MC3T3-E1细胞增殖的影响183.3金丝桃苷对MC3T3-E1细胞碱性磷酸酶活性的影响203.4金丝桃苷对MC3T3-E1细胞矿化能力的影响21RT-PCR结果223.5.1金丝桃苷对MC3T3-E1细胞成骨分化相关基因ALP、Col-Ⅰ和OPN表达的影响223.5.2金丝桃苷对MC3T3-E1细胞成骨分化相关基因BMP2、Runx2和Osx表达的影响223.5.3金丝桃苷对MC3T3-E1细胞成骨分化相关基因LRP5和β-catenin表达的影响23Western-blot结果233.6.1金丝桃苷对MC3T3-E1细胞成骨分化相关蛋白BMP2表达的影响·············