抗人igm抗体抑制鼻咽癌hne-抗人igm抗体抑制鼻咽癌hne.docxVIP

- - PAGE 10 - 组Survivin和PCNA阳性表达的结果显著低于IgG组和PBS对照组，差异有统 计学意义（P0.05）。 （2） Ang-2、MVD表达情况，抗人IgM抗体干预组 Ang-2表达的平均光密度值为（0.121±0.021），IgG组Ang-2表达的平均光 密度值为（0.188±0.013），PBS对照组的平均光密度为（0.196±0.005）， 实验组（抗人IgM抗体干预）Ang-2阳性表达的结果显著低于IgG组和PBS 对照组，差异有统计学意义（P0.05）；MVD表达情况，抗人IgM抗体干预 组MVD为（12.54±0.47），显著低于IgG组（16.95±0.21）及PBS对照组（17.17 ±0.47），统计学分析差异有统计学意义（P0.05）。结论：（1）抗人IgM抗 体可以显著抑制人鼻咽癌HNE-1细胞裸鼠移植瘤内Survivin、PCNA及Ang-2 的表达。（3）抗人IgM抗体对人鼻咽癌HNE-1细胞裸鼠移植瘤组织的新生 血管形成有明显的抑制作用，这可能与抗人 IgM 抗体通过抑制 Ang-2 及 Survivin的促血管生成的作用，拮抗IgM的生长因子样作用有关。（4） 抗 人IgM抗体可能成为鼻咽癌抗血管生成靶向治疗的新方向。 关键词：免疫球蛋白M；鼻咽癌；瘤；鳞状细胞；血管生成 Mechanism of anti-human IgM antibody on the tumorigenesis of human nasopharyngeal carcinoma HNE-1 cell in nude mice Abstract: Objective: With the progress of examination methods and treatments, The five years survival rate of Nasopharyngeal carcinoma has raised to to a certain extent. In our previous studies, we found that the immunoglobulin (Immunoglobulin, Ig) M is widely expressed in head and neck tumors, including nasopharyngeal carcinoma, With the intervention of anti-human IgM antibodies, it could significantly inhibit the growth of the tumorigenesis of human nasopharyngeal carcinoma HNE-1 cell in nude mice and promote apoptosis. Ig has growth factor-like role, but the exact mechanism is still not clear. With the depth study of on the mechanism of tumor formation, It was discovered that angiogenesis is closely related to growth and metastasis of tumors. Anti-angiogenesis therapy has also become an important part of the treatment of tumors. This study was on the basis of our preliminary studies, through the establishment of the HNE-1 cell xenografts in nude mice, we want to further study the biological activity of ectopic expression of IgM antibody on the tumorigenesis, to provide new ideas on clinical treatment of NPC. Methods: Take tumor tissue of transplanting human NPC HNE-1 cells in Balb/c-nu/nu nude mice embedded in paraffin, cut into 4μm thick slices. The expression of