抗人类成纤维细胞生长因子受体2(fgfr2)的单链抗体的筛选-screening of single chain antibodies against human fibroblast growth factor receptor 2 ( fgfr 2 ).docxVIP
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抗人类成纤维细胞生长因子受体2(fgfr2)的单链抗体的筛选-screening of single chain antibodies against human fibroblast growth factor receptor 2 ( fgfr 2 )
缩略语bp(basepair):碱基对cDNA(complementaryDNA):与mRNA互补的DNAcfu(colony-formingunit):菌落形成单位CDR(complementarydeterminingregion):互补决定区DMEM(dulbecco’smodifiedeagle’smedium):达氏修正依代培养基DMSO(dimethylsulfoxide):二甲基亚砜DNA(deoxyribonucleicacid):脱氧核糖核酸E.coli(escherichiacoli):大肠杆菌dNTP(deoxynucleosidetriphosphate):脱氧核苷三磷酸FBS(fetalbovineserum):胎牛血清FGFR2(fibroblastgrowthfactorreceptor2):成纤维细胞生长因子受体FGFs(fibroblastgrowthfactor):成纤维细胞生长因子LB(luriabrothmediumLuria):肉汤培养基PBS(phosphatebuffersolution):磷酸缓冲液PCR(polymerasechainreaction):聚合酶链反应PEG(polyethyleneglycol):聚乙二醇RNA(ribonucleicacid):核糖核酸scFv(single-chainvariablefragment):单链抗体EDTA(ethylenediaminetetraaceticacid):乙二胺四乙酸目录摘要......................................................................................................................................................IAbstract............................................................................................................................................II缩略语..............................................................................................................................................III目录....................................................................................................................................................IV第一章前言......................................................................................................................................11.1FGFR2简介11.2FGFR2与癌症之间的关系1FGFR2与胃癌1FGFR2与乳腺癌1FGFR2与胰腺癌2FGFR2与子宫癌2FGFR2与卵巢癌3FGFR2与肺癌31.3单链抗体及其应用.............................................................................................................31.4酵母双杂交技术简介.........................................................................................................41.5课题意义.............................................................................................................................6第二章技术路线简图......................................................................................................................7第三章实验材料和方法.........
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