口腔扁平苔藓患者外周血pd-pd in peripheral blood of patients with oral lichen planus.docxVIP

P1=0.007;r2=-0.365，P2=0.009；r3=0.482,P3=0.000）;sPD-L1表达量与CD4+存在负相关关系,与IgG存在正相关关系（Spearmanr1=-0.286，P1=0.044,r2=0.365，P2=0.029）；结论：OLP患者PD-1、PD-L表达升高提示PD-1/PD-L1信号介导负性调节信号过强，导致细胞免疫功能受到抑制甚至丧失，同时诱发机体体液免疫功能紊乱:同时,sPD-1、sPD-L1蛋白可在一定程度上通过影响PD-1/PD-L1信号通路参与抑制途径的调节作用,可能在OLP免疫发病机制中也起着一定作用。【关键词】口腔扁平苔藓;程序性死亡受体1;程序性死亡配体1;可溶性程序性死亡受体1;可溶性程序性死亡配体1;荧光定量PCR;酶联免疫吸附测定VTherelationshipbetweentheexpressionofPD-1/PD-L1andimmunestatusinpatientswithorallichenplanusPostgraduateLUOLiangSupervisorProf.CAIYang,[Abstract]Objective:TopreliminarystudythepossibleroleandsignificanceofPD-1/PD-L1pathwayinthepathogenesesofOralLichenPlanus(OLP).Methods:36casesofpatientswithOralLichenPlanus(20casesofreticularOLPand16casesoferosiveOLP)and18casesofhealthypeopleservedascontrols.Lymphocytesubsets(CD3+、CD4+、CD8+、CD19+、CD16++56+)ofperipheralbloodinpatientswereinvestigatedbyflowcytometricanalysis.Nephelometryimmunoassaywasperformedhumoralimmunity(IgG,IgA,IgM,C3,C4)inpatients.PD-1mRNAandPD-L1mRNAofperipheralbloodmononuclearcells(PBMCs)wereinvestigatedbyquantitativePCRandthelevelsofsPD-1,sPD-L1proteinsinserumofpatientswereinvestigatedbyELISA.ThecorrelationsbetweentheexpressionofPD-1,PD-L1,sPD-1,sPD-L1andtheimmunestatusandclinicalcharacteristicsofpatientswithOLPwereanalyzedbySPSSResults:1.CD3+、CD4+、CD8+、CD16++56+ofperipheralbloodinpatientswithOLPweredecreasedcomparedwiththenormalvalue,whileCD19+wasincreasedcomparedwiththenormalvalue(p0.05);HumoralimmunityinC3andC4werelowerlevels,however,elevatedinIgMlevels,thedifferenceswerestatisticallysignificant(p0.05).2.BothPD-1mRNAandPD-L1mRNAofperipheralbloodmononuclearcells(PBMCs),andsPD-1andsPD-L1proteinsofseruminpatientswithOLPweresignificantlyhigherthanthatincontrolgroup(p0.05);TherewasapositivecorrelationbetweenthelevelsofPD-1mRNAandsPD-L1proteins（Spearmanr=0.566，P=0.022).TherewerepositivecorrelationsbetweenthelevelsofPD-L1mRNAandsPD-1orsPD-L1proteins（Spearmanr1=0.537,P1=0.048;r2=0.582，P2=0.029）.3.PD-1mRNAexpressionlevelshasainversecorrelationbetweenthepresenceofCD4+,the