老鹰茶总黄酮中黄酮类单体对tgf-β1诱导的肝星状细胞增殖的影响及部分机制分析-effects of flavonoid monomers in total flavonoids of camellia oleifera on tgf - β 1 - induced proliferation of hepatic stellate cells and analysis of some mechanisms.docxVIP

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老鹰茶总黄酮中黄酮类单体对tgf-β1诱导的肝星状细胞增殖的影响及部分机制分析-effects of flavonoid monomers in total flavonoids of camellia oleifera on tgf - β 1 - induced proliferation of hepatic stellate cells and analysis of some mechanisms.docx

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老鹰茶总黄酮中黄酮类单体对tgf-β1诱导的肝星状细胞增殖的影响及部分机制分析-effects of flavonoid monomers in total flavonoids of camellia oleifera on tgf - β 1 - induced proliferation of hepatic stellate cells and analysis of some mechanisms

flavonidsoflitseacoreanaofhepaticfibrosisantioxidantmechanism.Specificcontentincludesthefollowingtwoparts:StudiesontheextractionandseparationoffourflavonoidsmonomerfromLitseaCoreanaL.Withtheflavonoidscontentasaevaluationindex,orthogonaldesignL9(33)isappliedtooptimizethewaterextractiontechniquefortotalflavonoidsofLitseaCoreanaL.Thebestextractiontechnologyisthat12BVwater,1hourofreflux,and3timesofextraction.TheD101macroporousresin(non-polartype)isthesuitablemediumtoprepareTFLC.Afteradsorptiononmacroporousresinanddesorptionbyethanol,thecontentoftotalflavonoidsinLitseaCoreanaLis48.59%,andtherecoveryrateis90%.Inthispaper,fourflavonoidsareseparatedfromTFLCbythenormal-phaseandreversed-phasesilicagelcolumnchromatography.Comparedwiththeknowncompounds,theyareidentifiedas:quercetin-3-O-?-D-galactoside,quercetin-3-O-?-D-glucoside,kaempferol-3-O-?-D-galactoside,andkaempferol-3-O-?-D-glucoside.StudiesontheeffectsandmechanismsofflavonoidsmonomerontheproliferationofhepaticstellatecellsinducedbyTGF-?1Thearticleadoptstheliverperfusiontoisolatethenormalrathepaticstellatecells,andcultureit.Thisseparationofhepaticstellatecellratioof2x107/liver,thecellvitalityandpurityismorethan90%.ByMTTmethodfordetectingfourflavonoidsmonomerproliferationofinhibitiononHSC,theresultshowsthat,aftercultivating24、48、72hour,fourkindsofflavonoidsmonomerhavevaryinglevelsofinhibitionontheproliferationofHSC,butkaempferol-3-O-?-D-glucosidehavethestrongesteffectiveat48h.Reversetranscription-polymerasechainreaction(RT-PCR)analysisofkaempferol-3-O-?-D-glucosideonHSCwhichwasinducedbyTGF-?1wasdetectedincollagenⅠ,collagenⅢ,Smad2,Smad3andSmad7receptormRNAexpressionintheimpact.itcanobviouslydown-regulatecollagenⅠ,collagenⅢ,Smad2andSmad3receptormRNAexpression,anditcanup-regulateSmad7mRNAexpression.KeywordsTotalflavonoidsofLitseacoreanaL;Orthogonaldesign;Liverperfusion;Hepaticstellatecells;Transforminggrowthfactor-beta1;TGF-?/Smadpathway英文缩略词Abbreviation英文缩写英文全称中文全称Aabsorbance吸光度BVresinbedvolume树脂床体积Cconcentration浓度CCl4carbo