雷公藤多甙及雌孕激素治疗对大鼠卵巢bcl-effects of tripterygium wilfordii polyglycoside and estrogen and progesterone on rat ovary bcl.docxVIP
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雷公藤多甙及雌孕激素治疗对大鼠卵巢bcl-effects of tripterygium wilfordii polyglycoside and estrogen and progesterone on rat ovary bcl
observedafterHEstaining.UsingtheimmunohistochemicalstainingtechniquewedetectedtheexpressionlevelofapoptosisregulatoryproteinBcl-2andBaxinovariangranularcell.ResultsComparedwiththecontrolgroup,estrogenreplacementtherapycombinedwithprogestogencouldrestrainthesepathologicalchangesproducedbyTWP,ThelevelofE2secretedbyovarywaslowerandthelevelofLHandFSHsecretedbypituitarywashigherintheTWPgroupandrecoverygroupthanthecontrolgroup(P0.05).Andtherewasnosignificantdifferencebetweenthecontrolgroupandtheinterventiongroup(P0.05).TotheeffectoftheBcl-2expressionsinovarycellsoftherats,theTWPgroupwaslowerthanthecontrolgroup,andtheinterventiongroupandthetreatmentgroupwereobviouslyhigherthantheTWPgroup,inthesametimetheexpressionofBaxproteinshowedthecontrarychange(P0.01),amongthetotal,therewasnosignificantdifferencebetweenthecontrolgroupandtheinterventiongroup(P0.05).ConclusionHPTcanpreventovaryinjuryproducedbyTWP,whosemechanismmaybeatleastpartlycorrelatedwithup-regulationondecreasedexpressionofBcl-2andsuppressiononincreasedexpressionofBax[Keywords]Tripterygiumwilfordii;Genitalsystem;Hormonereplacementtherapy;Bcl-2andBax英文缩略词表英文缩写英文全名中文全名E2FSHGCshHEHCGHPTLHminmRNANF-κBPPMSGRIASTWPTWWApaf-1ethidiumbromideFollicle-stimulatinghormonegranulosacellshourhematoxylin-eosinhumanchorionicgonadotropinhormonereplacementtherapyluteinizinghormonemimutemessengerribnucleicacidnuclearfactor-κ-bindingprogesteronepregmnantmareserumgonadotropinradio-immunityapproachsecondtripterygiumwilfordiipolyglycosidiumtripterygiumwilfordiiweekapoptoticprotease–activatingfactor-1雌激素卵泡刺激素卵巢颗粒细胞小时苏木素-伊红人绒毛膜促性腺激素激素替代治疗黄体生成素分钟信使核糖核酸核转录因子-κB孕酮孕马血清促性腺激素放射免疫法秒雷公藤多甙雷公藤周凋亡蛋白酶激活因子1学位论文独创性声明本人所呈交的论文是我个人在导师指导下进行的研究工作及取得的研究成果。据我所知,除了文中特别加以标注和致谢的地方外,论文中不包含其他人已经发表或撰写过的研究成果。与我一同工作的同志对本研究所做的任何贡献均已在论文中作了明确说明并表示谢意。学位论文作者签名:日期:学位论文使用授权声明本人完全了解安徽医科大学有关保留、使用学位论文的规定:学校有权保留学位论文并向国家主管部门或其指定机构送交论文的电子版和纸质版,有权允许论文进入学校图书馆被查阅,有权将学位论文的内容编入有关数据库进行检索,有权将学位论文的标题和摘要汇编出版。愿意将本人的学位论文提交《中国博士学位论文全文数据库》、《中国优秀硕士学位论文全文数据库》和《中国学位论文全文数据库》中全文发表,并可以以电子、网络及其他数字媒体形式公开出
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