kiss1和gpr54基因在骨肉瘤中表达的研究-expression of kiss 1 and gpr 54 genes in osteosarcoma.docxVIP

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kiss1和gpr54基因在骨肉瘤中表达的研究-expression of kiss 1 and gpr 54 genes in osteosarcoma.docx

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kiss1和gpr54基因在骨肉瘤中表达的研究-expression of kiss 1 and gpr 54 genes in osteosarcoma

福建医科大学福建医科大学 2011 届硕士研究生毕业论文 6 6 expression, and the difference was significant. 5. GPR54 mRNA and protein expressed strongly in all the 3 types of osteosarcoma cell lines and normal osteoblast cell line hF-OB 1.19, but no significant difference existed between each other. 6. Patients with GPR54 protein positive expression had a shorter median survival time than patients with GPR54 protein negative expression, and the difference was significant. 7. Patients with both KiSS-1and GPR54 protein positive expression had a higher mortality rate than patients with both KiSS-1and GPR54 protein negative expression, and the difference was significant. [Conclusions] 1. The expression level of KiSS-1 mRNA and protein in normal osteoblast cell is significant higher than in 3 types of osteosarcoma cell. With the gradually increased aggressive ability, the expression level of KiSS-1 mRNA and protein decreased in the 3 types of osteosarcoma cell lines, which indicate KiSS-1 gene maybe play a role of supression of metastasis in osteosarcoma cell lines. 2. The expression level of KiSS-1 is higher in osteosarcoma patients than in benign osteochondroma patients, the expression level of KiSS-1 is positve correlation with the metastasis and negative correlation with the survival time in osteosarcoma patients. 3. GPR54 mRNA and protein expressed strongly in all the 3 types of osteosarcoma cell lines and normal osteoblast cell line hF-OB 1.19, but no significant difference existed between each other. 4. The expression level of GPR54 is higher in osteosarcoma patients than in benign osteochondroma patients, and the expression level of GPR54 is negative correlation with the survival time in osteosarcoma patients. Both KiSS-1and GPR54 protein positive expression is negative correlation with the survival time in osteosarcoma patients. [Key Words] osteosarcoma ；KiSS-1；GPR54 1 1 学位论文原创性声明和版权使用授权书学位论文原创性声明本人郑重声明：所呈交的学位论文，是本人在导师的指导下，独立进行研究工作所取得的真实成果。除文中已注明引用的内容外，本论文不含任何其他个人或集体已经发表或撰写过的作品成果。对本