芍药甘草附子汤对类风湿性关节炎大鼠免疫调节作用.docVIP

芍药甘草附子汤对类风湿性关节炎大鼠免疫调节作用 　　摘 要：目的：探讨芍药甘草附子汤对类风湿性关节炎的作用机理。方法：48只Wistar大鼠随机分为空白对照组、模型组、雷公藤多甙组、芍药甘草附子汤组。除空白组外，各组大鼠复制类风湿关节炎模型，连续灌胃给药21天，采血测定白介素-1β（IL-1β）、前列腺素E2（PGE2）水平，取左踝关节组织观察病理改变。结果：芍药甘草附子汤组血清中IL-1β、PGE2明显降低，与模型组比较有显著性差异。关节病理损害明显改善。结论：芍药甘草附子汤能够降低IL-1β、PGE2含量从而减轻其对关节的侵害。 　　关键词：芍药甘草附子汤；类风湿性关节炎；IL-1β；PGE2 　　中图分类号：R285.5 　　文献标识码：A文章编号： 　　1673-7717（2008）05-1081-03 　　?? 　　Immunoregulatory Effects of PGAD on Rheumatoid Arthritis rats 　　?? 　　CHEN Rui,LIN Da?yong,FU Yong?qiang,advisor:GUAN Qing?zeng,GU Song?（ ??　Liaoning University of TCM, Shenyang 110032,Liaoning,China 　　）? 　　Abstract：Objective: To investigate the mechanism of the therapy of Paeoniae and Glycyrrhizae and Aconiti Decoction(PGAD)on the rheumatoid arthritis. Methods: Forty-eight Wistar rats were divided into normal comparison group, model group, Tripterygium Wilfordii Hook．f group and PGAD group at random. Except the normal comparison group, the others were fabricated Adjuvant Arthritis（AA）model. After treated the model rats for 21 days. Collected the blood of all rats to measure the level of Interleukin-1β(IL-1β) and Prostaglandin E2(PGE2).And histopathology change of left malleolus joint were observed through light microscope. Results: PGAD can reduce the content of IL-1β and PGE2,And relive the histopathology change of malleolus joint, which were statistically significant compared to those of model group. Conclusion: PGAD can reduce the content of IL-1β and PGE2 to lessen their destroy of the joint. 　　 　　Keywords：Paeoniae and Glycyrrhizae and Aconiti Decoction (PGAD);Rheumatic Arthritis; IL-1β；PGE2 　　 　　芍药甘草附子汤出自《伤寒论》，见于68条“发汗，病不解，反恶寒者，虚故也，芍药甘草附子汤主之。”由白芍、炙甘草、炮附子3味药组成，其中芍药酸苦，养血敛阴，柔肝止痛；甘草甘温，补中缓急，止痛；二药相合酸甘化阴，滋阴养血，缓急止痛；附子大辛大热，补火助阳，散寒止痛，与甘草相合辛甘化阳，附子性猛，得甘草而缓，芍药性寒，得附子而和。且芍药可破阴结，祛除经络中之瘀，甘草补中坚筋骨，附子走而不守，通脉散寒。本方虽小，但用药精炼，用治痹证包含祛除病因，改善临床症状等功能。并且毒副作用较小，易长期坚持服用。为探讨该方的作用机理，以其对弗氏完全佐剂关节炎(AA)大鼠的免疫调节及关节病理形态的影响为主要研究内容进行实验观察。? 　　 　　1实验材料? 　　 　　1．1实验动物 　　普通级健康Wistar大鼠48只［辽宁中医药大学动物实验中心提供，许可证号：SYXY（辽）2003-0019］