RP-HPLC法测定感冒清热颗粒荆芥穗薄荷中总胡薄荷酮含量.docVIP

RP-HPLC法测定感冒清热颗粒荆芥穗薄荷中总胡薄荷酮含量 　　[摘要] 目的：建立以RP-HPLC法测定感冒清热颗粒荆芥穗、薄荷中总胡薄荷酮含量的方法。方法：采用Phenomenex Gemini 5u C18 110A色谱柱（4.6 mm×250 mm，5 μm），流动相为：甲醇-水（50∶50），检测波长252 nm，柱温为30℃，流速为1.0 ml/min。结果：胡薄荷酮进样量在0.010 12～0.005 060 μg范围内有良好线性关系，平均加样回收率为99.74%，RSD为0.53%。3批样品中总胡薄荷酮的含量分别为0.063 18、0.063 24、0.063 22 mg/g。结论：本方法灵敏、准确，专属性强，重现性好，可作为感冒清热颗粒荆芥穗、薄荷中总胡薄荷酮的含量测定方法。 　　[关键词] 感冒清热颗粒；胡薄荷酮；含量测定 　　[中图分类号] R917 [文献标识码]A[文章编号]1673-7210（2011）07（b）-094-02 　　 　　Content determination of pulegone of schizonepeta spike and mentha haplocalyx in Ganmaoqingre Granule by RP-HPLC 　　LIU Lanjun1, SONG Weifeng2 　　1.Lanshi Hospital of Chancheng District, Foshan City, Foshan528041,China; 2.The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630 　　[Abstract] Objective: To establish the method for determination of pulegone of schizonepeta spike and mentha haplocalyx in Ganmaoqingre Granule. Methods: The analytical column Phenomenex Gemini 5u C18 110A (4.6 mm×250 mm, 5 μm) was used. The mobile phase was composed of methanol-water (50∶50) at a flow rate of 1.0 ml/min. The wavelength of UV detector was 252 nm and column temperature was 30℃. Results: The linear ranges of pulegone was 0.010 12-0.005 060 μg. The average recovery was 99.24% with RSD of 0.53%. The total contents of pulegone in three batches of samples were 0.063 18, 0.063 24, 0.063 22 mg/g respectively. Conclusion: This method is sensitive, accurate, exclusive. It can be applied to determine the content of pulegone of schizonepeta spike and mentha haplocalyx in Ganmaoqingre Granule. 　　[Key words] Ganmaoqingre Granule; Pulegone; Content determination 　　 　　 　　感冒清热颗粒由荆芥穗、薄荷、防风、柴胡、葛根、苦地丁等组成，具有疏风散寒，解表清热的作用，用于风寒感冒，头痛发热，恶寒身痛，鼻流清涕，咳嗽咽干等。其标准收载在《中国药典》2010年版，原来质量标准有葛根中葛根素的含量测定方法[1]185。方中君药荆芥穗具有散风湿、利咽喉???解表散风、透疹的作用[2]，用于感冒、头痛、麻疹、风疹，疮疡初起。其有效成分胡薄荷酮具有抗炎的作用[3]。有报道[4-5]对其中的防风、葛根进行升麻苷、5-O-甲基维斯阿米醇苷和葛根素的含量测定，但未见有报道君药荆芥穗的含量测定。荆芥穗和薄荷均含有胡薄荷酮成分，为能有效控制制剂中总胡薄荷酮的含量，本文现参照《中国药典》2010年版荆芥穗的含量测定方法[1]217，采用高效液相色谱法测定荆芥穗和薄荷中总胡薄荷酮的含量，并对本实验方法进行方法学的研究，为更有