类风湿关节炎患者外周血白细胞介素-23和类风湿因子表达研究.docVIP

类风湿关节炎患者外周血白细胞介素-23和类风湿因子表达研究 　　[摘要] 目的：检测类风湿关节炎（RA）患者体内白细胞介素（IL）-23和类风湿因子（RF）的表达，探讨IL-23在RA发病中的作用及其与RF的关系，为RA的早期诊断和进一步阐明RA的发病机制提供理论依据。方法：采用逆转录-聚合酶链反应（RT-PCR）检测66例RA患者和44例健康对照者外周血单个核细胞（PBMC）中IL-23p19 mRNA的表达水平，采用酶联免疫吸附试验（ELISA）检测血清IL-23浓度，采用免疫比浊法检测血清RF的浓度。结果：RT-PCR显示RA患者PBMC中IL-23p19 mRNA的表达[（45.2±11.7）%]显著高于健康对照组[（23.8±9.5）%]，ELISA检测结果显示RA患者血清IL-23水平[（114.47±42.70）ng/L]显著高于健康对照组[（70.81±28.85）ng/L]，差异均有统计学意义（均P＜0.05）。RA患者血清IL-23水平与RF含量无明显相关性（r=0.389，P＞0.05）。结论：RA患者体内IL-23在基因和蛋白水平均过表达，提示IL-23可能参与了RA的免疫病理过程，在RA的发病中发挥作用。 　　[关键词] 类风湿关节炎；白细胞介素-23；类风湿因子 　　[中图分类号] R593.22 [文献标识码] A [文章编号] 1673-7210（2011）06（c）-023-03 　　 　　The study of interleukin-23 and rheumatoid factor expression in peripheral blood of patients with rheumatoid arthritis 　　ZHNAG Guohua1,2, LU Zhi1* 　　1.Second Clinical Medical College, Shanxi Medical University, Shanxi Province, Taiyuan 030001, China; 2.Fenyang College of Shanxi Medical University, Shanxi Province, Fenyang 032200, China 　　[Abstract] Objective: To detect the expression of interleukin (IL)-23 and rheumatoid factor (RF) in rheumatoid arthritis (RA) patients and to explore the role of IL-23 in the pathogenesis of RA and its relationship with the RF, for early diagnosis of RA and further clarify the pathogenesis of RA provides theoretical basis. Methods: IL-23p19 mRNA expression in peripheral blood mononuclear cells (PBMCs) of 66 RA patients and 44 healthy controls was detected by reverse transcription-polymerase chain reaction (RT-PCR), serum IL-23 concentration were measured by enzyme-linked immunosorbent assay (ELISA), serum RF concentrations were measured by immunoturbidimetry. Results: RT-PCR showed IL-23p19 mRNA expression in PBMC of RA patients [(45.2±11.7)%] was significantly higher than that of healthy controls [(23.8±9.5)%], ELISA results showed IL-23 concentration in serum of RA patients [(114.47±42.70) ng/L] was significantly higher than that in healthy controls [(70.81±28.85) ng/L], the differences were significant (P