蛋白相互作用的研究方法.pdfVIP

Thermo Scientific Pierce 蛋白相互作用的研究方法 罗 莎 Rosa Luo Ph.D. Application Scientist Biosciences Division Thermo Fisher Scientific China 酵母蛋白质相互作用图谱 Thick blue lines represent literature-derived interactions from PreBIND+MIPS in the HMS-PCI dataset. Thin orange lines represent potential novel interactions. Courtesy MDS Proteomics 2 Proprietary Confidential 蛋白质相互作用技术 Genetic Chemical  Two Hybrid  Crosslinking  Phage Display  Label-transfer  Mutational analysis  FeBABE mapping Biochemical Fluorescent  Immunoprecipitation (IP)  Immunofluorescence colocalization  Co-Immunoprecipitation (Co-IP)  FRET (Fluorescence Resonance  Pull-Down Assays Energy Transfer)  Far Western 3 Proprietary Confidential General Purification of Proteins and Interacting Proteins • Immunoprecipitation (IP) • Co-IP • Pull-Down Assays 4 Proprietary Confidential 蛋白相互作用研究方法 • 免疫沉淀(IP) • 富集、纯化、检测兴趣蛋白 • 用抗体捕获抗原 Prey • 免疫共沉淀(Co-IP) • 富集、纯化、检测兴趣蛋白 • 用抗体捕获抗原和互作蛋白 Bait Prey • Pull-Down • 确定蛋白之间的互作 • 用标签蛋白捕获结合蛋白 Bait Prey 5 Proprietary Confidential