不同分离技术对秦七风湿方水提液物理化学参数影响-influence of different separation technology on physicochemical parameters of water extract from qinqi fengshi recipe.docxVIP
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不同分离技术对秦七风湿方水提液物理化学参数影响-influence of different separation technology on physicochemical parameters of water extract from qinqi fengshi recipe
ofcommonmacromolecule,respectivelyusingtheKaumasblueG-250colorimetricmethod,hidepowdermethod,weightdeterminationmethod,enzymehydrolysis-potassiumpermanganatemethod;ChikusetsusaponinⅣaandGentiopicrosideandLoganinformajorcomponents,respectivelyusingHPLCmethodforquantitativeresearch;usingTLCmethodforqualitativeresearch;byHPLCfingerprinttechnologyanalysisofthecharacteristicsofeachseparateunitofcomposition.Results1,Throughthesinglefactorexperimentinvestigationofdecoctingtime,wateramount,decoctiontimesastheindexcomponentcontentinQinqiRheumatism,theoptimumextractionconditionsweredeterminedasfollows:extractiontime120min,quantityofwater16times,3timesofDecoction.2,Useappropriatemethodsandequipmenttoremovetheqinsevenrheumatismextractimpurities,improvethepurityofactiveingredient,obtainedthebestmacroporousresinseparationprocessparameters:D101macroporousresin,ratiooftwoadsorptionresinandmedicinalmaterials,onthesampleconcentrationis0.2mg/mLofliquids,resindiameterratioof1:10,attherateof1bv/honsampleanduse4bvfirstwashed,reoccupy4bv30%ethanolelutioncollection,with3bv95%ethanolelutionfinallycollected.Forquarter,energysavingandtomaximizetheretentionofeffectivecomponentsofQinqiRheumatismsquarewaterextractofpurpose,finalchoiceofoperatingconditionswere:membraneporesizeof0.2μm,theoperationpressureis0.35MPa,theliquiddrugconcentrationwas0.1g/mL,afilmatatemperatureof30℃DifferentmodernseparationtechnologyinQinqiRheumatismeffectbetweenthephysicalandchemicalcharacterizationAffectthecorrelationbetweenparameters,andapreliminaryscreeningofmembraneseparationforabetterseparationtechnology.ConclusionThispapershowsthat,usingdifferentmodernseparationtechnology(macroporousresinseparationtechnology,membraneseparationtechnology)inQinqiRheumatismeffectbetweenthephysicalandchemicalcharacterizationAffectthecorrelationbetweenparameters,andapreliminaryscreeningofmembraneseparationKeywords:QinqiRheumatism;macroporousresinseparation;membraneseparation;commonmacromolecule,;physicalandchemicalcharacterizationAuthor
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