番茄花药和小孢子离体培养及转化雄性不育基因barnase的研究-study on anther and microspore culture in vitro and transformation of male sterile gene barna se in tomato.docxVIP

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2018-05-29 发布于上海
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番茄花药和小孢子离体培养及转化雄性不育基因barnase的研究-study on anther and microspore culture in vitro and transformation of male sterile gene barna se in tomato.docx

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番茄花药和小孢子离体培养及转化雄性不育基因barnase的研究-study on anther and microspore culture in vitro and transformation of male sterile gene barna se in tomato

AbstractTomato(LycopersiconesculentumMill)belongedtoSolanaceaeLycopersicon,widelycultivatedthroughouttheworld.However,progressinbreedingnewvarietiesonlybyconventionalbreedinghadbeenslowed.Productionofhaploidsintomatoantherormicrosporeculturewouldallowtomatobreedertoreleasenewlinesmorequicklyandscreenforparticulartraitsmoreefficiently.Atthesametime,tomatowasself-pollinationcrop,itsheterosiswasveryclearly.Thestudyonmalesterilityoftomatowasveryimportant.Inthispaper,anthercultureandisolatedmicrosporecultureintomatowerestudied.Andregeneratedplantswereobtainedfrom‘Liger87-5’,‘Sf9’and‘Hp’throughanther.LotsofGlobularembryoids,Heart-shapedembryoidswereproducedfrom‘Liger87-5’,‘Jg’,‘Zs’and‘Hp’throughisolatedmicrosporeforthefirsttime.Atthesametime,Agrobacterium-mediatedgenetictransformationsystemwasadopttedfortobaccoandtomato.Weobtained25trangenictobaccoand20transgenictomato.Specificresultswereasfollows:Weestablishedastableandhighefficientembryogeniccallusinductionandplantsregenerationsystemintomato.Sixtomatovarietieswereusedintheexperiment.Theresultsshowedthatthecalluswasrelatedtothegenotypes;Thecallusinducementfrequencywithadditionofpotatomilkandactivecarbondecreased35.14%、95.48%inthesixdifferentmaterialsrespectively.Theoptimizedhormonecombinationofcallusinducementwas1.0mg/L2,4-Dand0.5mg/LKT.30g/Lsucrosecouldenhancethecallusinducementabout20.24%on‘Liger87-5’.10μmol/Lsilvernitratecouldreducebrowningofthetissuesabout70.59%.Whentheconcentrationofsilvernitrateexceeded50μmol/L,thebrowningofthetissueswouldincreasedandthecallusinducementwoulddecreasedimperceptibility.MediumofMS+ZT1.5mg/L+IAA0.2mg/Lcouldmakecallusdedifferentiateintoadventitiousbud,andtherateofcallusinducementwas40%,thishormonefortomatoanthercallusdifferentiatedintoadventitiousbudsagainoptimumformula.Callusinductionintomatoshootthemediumwith2mg/Ltriacontanol,increasedtherateofbuddifferentiation,butalsocouldimprovethequalityofadventitiousbuds,adventitiousbudssignificantlyincreasedthepercentageofeffectivebud.Theadventit