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放线菌fim06-0063次级代谢产物的研究-study on secondary metabolites of actinomycete fim 06 - 0063
目录摘要..........................................................................................................................3Abstract4第一部分前言............................................................................................................61.1放线菌次级代谢产物的研究意义................................................................61.2美登木类化合物的国内外研究进展............................................................6第二部分放线菌 FIM06-0063 的发酵培养102.1仪器与材料..................................................................................................102.1.1仪器....................................................................................................102.1.2材料....................................................................................................102.2实验方法......................................................................................................102.2.1培养方法............................................................................................102.2.2高效液相色谱(HPLC)测定112.3结果与讨论.................................................................................................. 112.3.1最佳发酵周期的确定........................................................................ 112.3.2最佳装液量的确定............................................................................122.3.3扩大发酵............................................................................................12第三部分放线菌 FIM06-0063 次级代谢产物的分离纯化133.1仪器与材料..................................................................................................133.1.1仪器....................................................................................................133.1.2主要试剂及耗材................................................................................133.1.3常用显色剂........................................................................................133.2实验方法......................................................................................................143.2.1发酵产物的提取...........................................
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