pygo2对人osrc2肾细胞癌作用的动物实验分析-animal experimental analysis of py go2 on human os rc2 renal cell carcinoma.docxVIP

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pygo2对人osrc2肾细胞癌作用的动物实验分析-animal experimental analysis of py go2 on human os rc2 renal cell carcinoma.docx

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pygo2对人osrc2肾细胞癌作用的动物实验分析-animal experimental analysis of py go2 on human os rc2 renal cell carcinoma

第二章：英文摘要Animal experiments used to study the role of Pygo2 in human renal cell carcinoma OS-RC-2 cell lines[Objective]To research influence of Pygo2 in human renal cell carcinoma OS-RC-2 celllines. To provide tools for the study of the mechanism of human cell carcinoma and the targeted therapy.[Methods]Use human renal cell carcinoma cancer OS-RC-2 cell lines and after a series of different treatments of Pygo2, then grouped transplanted into nude mice model tumorigenicity through Pygo2 of kidney cancer pathogenesis in vivo. MTT method was used to detect the proliferation of OS-RC-2 cell.16 mice divided into four groups, each group of four, respectively, with the four treated renal cancer cell suspensions, Group A: blank control group of experimental control group GFP (original cells), B group Pygo2scrRNA (blank vector implant), C group of the expression group Pygo2OE, D group silent group Pygo2shRNA (using gene knockout technology tomake Pygo2 low expression), 4 .0 x10 6 renal cell carcinoma cell suspension 0.2mlplanted in nude front right armpit. Then the formation of transplanted tumors was studied. IHC detected that each tumor invasive protein (MMP-9, MMP-7 and VEGF). [Result]2 days cells into the logarithmic growth phase. A, B, C tumor rates were 100%, D composed of tumor was 75% (experiment was repeated twice). Pygo2OE group bean size transplanted tumors visible in three days, 7 days, the GFP and Pygo2scrRNA group touched subcutaneous the Summary, Pygo2shRNA 10 transplanted tumors. Group C tumor formation rate was significantly better than the other three groups (P0.05). Group D of the speed of tumor formation and the tumor were lower than in the other groups (P 0.05).IHC analysis revealed that pygo2shRNA reduced the levels of matrix metalloproteinase-7(MMP-7) and VEGF in the OS-RC-2 cells in vivo,however, the loss of Pygo2 can not obviously decreased the expression of MMP-9, compared with reduction of MMP - 7 and VEGF expression quantity phase. [Conclu