原发性肝细胞癌患者癌组织 癌旁肝组织及血清中hbv bcp区变异位点的研究-study on the mutation sites of hbv bcp region in liver tissue adjacent to cancer tissue and serum of patients with primary hepatocellular carcinoma.docxVIP

目录前言..............................................................................................................................1一、对象与方法...............................................................................................................41.研究对象........................................................................................................................................41.1病例组.................................................................................................................................41.2对照组.................................................................................................................................42.入组及排除标准............................................................................................................................42.1入组标准.............................................................................................................................42.2排除标准.............................................................................................................................43.实验材料........................................................................................................................................53.1主要试剂.............................................................................................................................53.2主要仪器.............................................................................................................................54.实验方法步骤................................................................................................................................64.1乙肝病毒基因组DNA提取64.2目的基因片段PCR的扩增74.3PCR产物电泳鉴定，目的片段的纯化84.4目的片段的克隆操作.......................................................................................................10二、结果....................................................................................................................12三、结论.............................................................................................