HPLC法测定消渴降糖胶囊中槲皮素含量.docVIP

HPLC法测定消渴降糖胶囊中槲皮素含量 　　[摘要] 目的：探讨用高效液相色谱法检测消渴降糖胶囊中槲皮素的含量。方法：应用高效液相色谱法，采用C18柱，以甲醇-0.4%磷酸（55∶45）为流动相，以360 nm为检测波长，检测消渴降糖胶囊中槲皮素的含量。结果：槲皮素峰与相邻色谱峰能有效分离，柱效较高，槲皮素对照品进样量在0.056～0.560 μg范围内与峰面积呈良好的线性相关，回归方程为Y=40 103.2X-561.4，相关系数r值为0.999 8；精密度RSD=0.9%；10 h稳定性试验RSD=1.10%；重复性试验结果显示，5份样品含量的变异系数RSD=1.30%；3个不同浓度的加样回收试验，平均回收率为98.7%，RSD=0.90%。结论：采用高效液相色谱法测定消渴降糖胶囊中槲皮素的含量，专属性强，结果可靠，重现性好，可用于消渴降糖胶囊中槲皮素的含量测定。 　　[关键词] 高效液相色谱法；消渴降糖胶囊；槲皮素 　　[中图分类号] R927.2 [文献标识码] A [文章编号] 1673-7210（2011）11（a）-078-03 　　 　　Determination of quercetin in Xiaoke Jiangtang Capsule by HPLC 　　FU Qianxia 　　Gong′an Health School in Jingzhou City, Hubei Province, Jingzhou 434300, China 　　[Abstract] Objective: To explore the method of high performance liquid chromatography (HPLC) in the determination of quercetin in Xiaoke Jiangtang Capsule. Methods: High performance liquid chromatography was employed to test the quercetin contents of Xiaoke Jiangtang Capsules. C18 column, mobile phase of methanol-0.4% phosphoric acid (55∶45) and detection wavelength of 360 nm were adopted. Results: The peak of quercetin and the adjacent peaks could be differentiated with high column efficiency. The quercetin reference standard sample size of 0.056 to 0.560 μg had a good linear correlation with the regression equation of Y=40 103.2X－561.4 and peak area (r=0.999 8), the precision RSD was 0.9% and the RSD of 10 hours stability test result was 1.10%. In repeated tests, the RSD of coefficient of variation among the 5 samples content was 1.30%; added sample tests of 3 different concentrations had average recovery rate of 98.7% and RSD was 0.90%. Conclusion: It can be concluded that, HPLC is stable and reliable in the determination of quercetin in Xiaoke Jiangtang Capsule. 　　[Key words] High performance liquid chromatography; Xiaoke Jiangtang Capsule; Quercetin 　　消渴降糖胶囊为《部颁标准??中药成方制剂第十五册收载品种，其标准号为WS3－B－2983－98。该质量标准颁布于1998年，仅涉及番石榴叶中所含槲皮素的薄层鉴别，无含量测定项目。为了更有效地控制产品质量，笔者在消渴降糖胶囊的质量标准中增加了槲皮素含量测定项目，以便对药品质量进行定性鉴别和定量测定双重控制，更有效地保证消渴降糖胶囊的内在质量。