不同运动方式对P53TIGARHKII基因表达和乳酸含量影响.docVIP

不同运动方式对P53TIGARHKII基因表达和乳酸含量影响 　　摘 要： 目的：比较不同训练方式对P53、TIGAR、HKII基因表达和乳酸含量的影响。方法:30 只大鼠随机分为安静组(C)、耐力训练组(E)、间歇性冲刺训练组(F)，训练6周。最后一次训 练结束后24～48 h内切取腓肠肌检测P53、TIGAR、HKIImRNA转录和P53、TIGAR的蛋白表达以 及乳酸含量。结果：1）E组、F组与C组相比、E组和F组相比P53mRNA转录、蛋白表达水平均 没有显著性差异。 2）E组TIGARmRNA和蛋白表达明显高于C组，而F组与C组相比、F组与E组 相比TIGARmRNA和蛋白表达均没有显著性差异。3）E组、F组与C组相比、E组和F组相比HKIIm RNA表达均没有显著性差异。4）E组与C组相比、E组与F组相比乳酸含量没有显著性差异，F 组乳酸含量非常显著高于C组。结论：1）两种训练方式都不能影响P53基因表达。2）耐力训 练可能抑制了糖酵解促进了线粒体的有氧呼吸。3）两种训练方式对血糖稳态的有力控制对 ???持机体正常生理功能发挥具有重要意义。4）耐力训练在一定程度上抑制了P53调节的糖酵 解信号通路，而间歇性冲刺训练似乎对该通路没有明显的影响。 　　关键词：耐力训练；间歇性冲刺训练；P53；TIGAR；HKII；乳酸 　　中图分类号：G804.7 文献标识码：A 文章编 号：1007-3612(2011)05-0060-04 　　Effects of Different Exercises on Gene Expression of P53 ?TIGAR?HKII and the Content of Lactic Acid 　　SHAO Yue 　　(Henan University of Economics and law， Zhengzhou 450002，Hena n China) 　　Abstract: Purpose: To compare gene expression of P53?TIGAR?HKII to different training pr ogram. Method: 30 rats were distributed into three groups: sedentary(C)，interv al sprint training (F) and endurance training (E). After 6 weeks，gastrocnemiuswas excised during 24～48h after the last treadmill test and analyzed.Result:1 ）there was no significant difference on P53 gene expression among E and C and Fgroup. 2) TIGARmRNA gene expression was increased in E group. There was no ma rked difference between F and C group， F and E group on both gene transcriptio n and translation. 3) There was no marked difference on HKII gene transcriptionamong E and C and F group. 4) There was a highly upregulation in F group versu s C group on the content of lactic acid， but no marked difference both in E ver sus C and E versus F group. Conclusion:1) Endurance training and sprint interva l training have no effect on P53 gene expression. 2) Endurance training mostlyinhibits the glycolysis signal pathway and facilitating aerobic respiration at t he same time. 3) The stronger control to blood sugar homeostasis of the two tra ining programs will be important to maintain the normal phy