大叶千斤拔活性成分分布及积累动态[权威资料].docVIP

大叶千斤拔活性成分分布及积累动态 　　[摘要] 目的 考察染料木苷、染料木素及大豆苷元在大叶千斤拔植株不同部位分布与积累的动态变化规律。 方法 利用高效液相色谱法，采用C18反相色谱柱，柱温为35℃，以甲醇-0.1%磷酸水溶液（48∶52，V/V）为流动相，检测波长为250 nm，测定和比较不同采收期大叶千斤拔根、茎、叶中染料木苷、染料木素和大豆苷元的含量以及不同生长年限的大叶千斤拔根中三种成分的含量。 结果 染料木苷、染料木素和大豆苷元主要分布于大叶千斤拔根中，且在10月份积累量最高，2月份积累量最低；6.5～7.5年生大叶千斤拔根中这三种成分的含量最高，但从种植成本考虑，3.5年采收比较合适。 结论 根据大叶千斤拔中活性成分的分布与积累规律，发现大叶千斤拔的最佳采收期为10月份，最佳采收年限为三年半，最佳药用部位为根。 　　[关键词] 大叶千斤拔；染料木素；染料木苷；大豆苷元；分布；积累动态 　　[] R282.6 [] A [] 1673-7210（2016）10（a）-0017-04 　　Distribution and accumulative dynamic variation of bioactive components in Moghania macrophylla 　　NIU Yingfeng LI Xiaohua LI Haitao ZHANG Zhonglian GUAN Yanhong ZHANG Lixia▲ 　　Key Laboratory of Dai and Southern Medicine of Xishuangbanna Dai Autonomous Prefecture， Yunnan Branch of Institute of Medicinal Plant Development， Chinese Academy of Medical Sciences， Yunnan Province， Jinghong 666100， China 　　[Abstract] Objective To investigate the content and dynamic distribution of three active ingredients include genistein， genistin and daidzin in Moghania macrophylla. Methods The HPLC method was adopted， the chromatograph column was C18 spincolumn and the column temperature was 35℃， the mobile phase was methanol-0.1% phosphoric acid （48∶52， V/V）， and the detection wavelength was 250 nm. The content of genistin， genistein and isoflavoues aglycone in the root， steem and leaf of Moghania macrophylla which harvested in different periods were determinated， the content of the three active ingredients in the root of Moghania macrophylla which harvested in different year were determinated. Results The three active ingredients were mainly distributed in the root of Moghania macrophylla， the accumulation content of the three active ingredients were the highest in October and lowest in February， for different growth ages， the contents of the three active ingredients were the highest in the root of Moghania macrophylla which were 6.5-7.5 years old， but the 3.5 years old Moghania macrophylla was more appropr