局部注射neuritin促进骨折愈合的实验分析-experimental analysis of local injection of neur itin to promote fracture healing.docxVIP

目的:中文摘要通过局部注射Neuritin观察neuritin在SD大鼠骨折模型中促进骨折愈合的作用，并可能为临床治疗骨不连和骨缺损开拓新思路和提供理论依据。方法:将96只健康雄性SD大鼠随机分为实验组和对照组，实验组72只，对照组24只。各组动物取右侧股骨，切断中段股骨后行克氏针髓内固定术制作骨折模型。对照组不予特殊处理。实验组根据用药剂量实验组分为A、B、C3组，各组分别每日给予neuritin0.1μm/L、0.05μm/L、0.025μm/L局部注射治疗，并分别于1周、2周、4周处死，所得标本进行拍摄X线片、测量骨痂体积、做病理切片等处理。所得数据应用SPSS17.0统计软件进行分析处理，不同组间比较采用x2检验，计量资料的各项指标均采用均数±标准差（x??s）表示，组间比较采用单因素方差分析，分析前行方差齐性检验及正态检验。以α=0.05为检验水准，P0.05为有统计学意义。结果:①大体标本观察及X线拍片显示：实验组各组与同期对照组比较，骨折愈合骨化速度明显增快，与对照组之间差异明显(P0.05)；而A组显著快于C组大鼠（P0.05)。②骨痂体积测量结果显示：同期实验组与对照组比较骨痂体积明显增大，均明显大于对照组(P0.05)；实验各组之间，A组明显大于C组大鼠（P0.05)。③病理切片HE染色显示:实验各组在相同时间点内其软骨细胞、成骨细胞明显多于对照组(P0.05)。实验组之间比较，实验A组与B组、B组与C组之间差别具有统计学意义(P0.05)。结论:局部注射neuritin后可使动物骨折模型的骨痂含量增加，骨痂骨化速度增快。关键词neuritin；骨折动物模型；骨折愈合；局部注射论文类型：A(基础研究)AbstractObjective:ToobservetheeffectofneuritinonPromotingFractureinSD-ratsbylocalinjectionofneuritin,toexplorenewdruginterventionfornonunionandbonedefectsinclinicaltreatment.Method:96healthySDratswererandomlydividedintoexperimentalandcontrolgroup,72casesforexperimentalgroupand24casesforcontrolgroup.AllanimalsweremanufacturedtofracturemodelthroughbrakingrightmiddlefemurbyintramedullaryretrogradeKirschnerwirefixation.Controlgrouphadnospecialhandling.Experimentalgroups,namelygroupA、groupB、groupC,wererespectivelygivendailygavage0.1μm/L、0.05μm/L、0.025μm/Lneuritintreatment,respectively1-week,2-week,3-weekratswerekilled,takingX-ray,andsurveyingcallusvolume，manufacturingpathologicalsection.DatawereanalyzedusingStudent’st-testandexpressedasthemean±SEM.P0.05wasconsideredstatisticallysignificant.Result:SpecimensobservationandX-rayshows:theexperimentalgroupscomparedwiththecontrolgroup,fracturehealingcallusossificationratecontentandthereweresignificantdifferences,weresignificantlyfasterthanthecontrolgroup(P0.05);And,groupAwassignificantlyfasterthangroupC(P0.05);Callusvolumemeasurementshows:theexperimentalgroupcomparedwiththecontrolgroup,callusvolumeweresignificantdifferentandsignificantlylargerthanthecontrolgroup(P0.05);And,,groupAwassignificantlylargerthangroupC(P0.05);